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Updated: Nov 22, 2025
Biological Compatibility Profile on Biomaterials for Bone Regeneration
Published on: November 16, 2018
A bone substitute composed of polymethyl-methacrylate bone cement and Bio-Gene allogeneic bone promotes osteoblast
Zhikun Wang1,2, Zaixue Li2, Xiansen Zhang2
1Department of Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Background:
Increasing reports on new cement formulations that address the shortcomings of PMMA bone cements and various active components have been introduced to improve the biological activity of PMMA cement.
Objective:
Evaluating the biological properties of PMMA cements reinforced with Bio-Gene allogeneic bone.
Methods:
The MC3T3-E1 mouse osteoblast-like cells were utilized to determine the effects of Bio-Gene + PMMA on osteoblast viability, adhesion and differentiation.
Results:
The combination of allogeneic bone and PMMA increased the number of adherent live cells compared to both control group and PMMA or Bio-Gene group. Scanning electron microscopy observed that the number of cells adhered to Bio-Gene + PMMA was larger than Bio-Gene and PMMA group. Compared with the control and PMMA or Bio-Gene group, the level of ALP and the number of calcium nodules after osteoinduction was remarkably enhanced in Bio-Gene + PMMA group. Additionally, the combination of Bio-Gene and PMMA induced the protein expression of osteocalcin, osterix and collagen I.
Conclusion:
The composition of PMMA and allogeneic bone could provide a more beneficial microenvironment for osteoblast proliferation, adhesion and differentiation. PMMA bone cement reinforced with Bio-Gene allogeneic bone may act as a novel bone substitute to improve the biological activity of PMMA cement.
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