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Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

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A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
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Related Experiment Video

Updated: Nov 22, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
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PfAgo-based detection of SARS-CoV-2.

Fei Wang1, Jun Yang1, Ruyi He1

  • 1State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial BiotechnologySchool of Life Sciences, Hubei University, Wuhan, 430062, China.

Biosensors & Bioelectronics
|January 11, 2021
PubMed
Summary

A new molecular diagnostic platform, SARS-CoV-2 PAND, offers rapid and accurate COVID-19 screening. This Pyrococcus furiosus Argonaute (PfAgo)-based method significantly reduces detection time and instrument usage for large-scale testing.

Keywords:
Molecular diagnosisPfAgoSARS-CoV-2

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Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • Accurate and rapid COVID-19 diagnosis is critical for effective pandemic control.
  • Existing molecular diagnostic methods like RT-qPCR can be time-consuming and require specialized equipment.

Purpose of the Study:

  • To develop a highly sensitive and accurate molecular diagnostic platform for large-scale COVID-19 screening.
  • To improve the efficiency and accessibility of SARS-CoV-2 detection.

Main Methods:

  • Utilized Pyrococcus furiosus Argonaute (PfAgo) mediated nucleic acid detection.
  • Employed RT-PCR to amplify viral RNA from patient swabs, followed by PfAgo, guide DNA, and molecular beacon incubation.
  • Developed the SARS-CoV-2 PAND (PfAgo-based Nucleic Acid Detection) method.

Main Results:

  • The SARS-CoV-2 PAND platform demonstrated high sensitivity and accuracy, with 100% consistency with RT-qPCR for clinical samples.
  • Significantly reduced the Real-time PCR instrument usage time from over 1 hour to 3-5 minutes per batch.
  • Successfully identified the SARS-CoV-2 D614G mutant due to its single-nucleotide specificity.

Conclusions:

  • SARS-CoV-2 PAND is a rapid, sensitive, and accurate molecular diagnostic tool for COVID-19 screening.
  • The method alleviates the shortage of expensive Real-time PCR instruments, making large-scale screening more feasible.
  • PfAgo-mediated detection offers a promising alternative for infectious disease diagnostics.