Multiresidue Analytical Method for Pesticides in Soybean Extract

Developed and validated a fast, simple and effective method based on the use of DLLME technique and determination by GC-MS of 26 pesticides in SE. To carry out the extraction of the pesticides of the matrix, 70 μL of mix of pesticides (1.5 μg/mL) was added to 5.0 mL of SE, containing 1.0 g of sodium chloride and 3.0 mL of acetonitrile. The results of validation were suitable. The calibration curve was linear in the range of 0.500-5.0 μg/mL. The method showed a limit of detection and quantification of 0.17 μg/mL and 0.50 μg/mL, respectively. The recovery recovering between 47% and 115%, with relative standard deviation (RSD) of <20% for fortification levels (range of 1.0-3.0 μg/mL). The method validated can be applied for routine analysis in soy-based drinks, considering it is fast, easy to perform and has satisfactory validation results.