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From Microfluidic Paper-Based Analytical Devices to Paper-Based Biofluidics with Integrated Continuous Perfusion.

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Summary

This study introduces a novel, low-cost platform for continuous perfusion in microfluidic paper-based analytical devices (μPADs). This innovation enables μPADs for advanced biofluidic applications like cell culturing and drug screening.

Keywords:
cell cultureconcentration gradientcontinuous perfusiondrug screeningmicrofluidic paper-based analytical devices (μPADs)paper-based biofluidics

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Microfluidic paper-based analytical devices (μPADs) are facile tools for analytical and biomedical applications.
  • Current μPADs lack continuous perfusion control, limiting their use in biofluidics fields like cell culturing and drug screening.
  • Existing limitations hinder the full potential of paper-based devices in continuous flow applications.

Purpose of the Study:

  • To design and develop a novel, low-cost, and compact platform for controlling continuous perfusion in μPADs.
  • To overcome the limitations of existing μPADs for continuous flow applications.
  • To enable μPADs for advanced biofluidic applications.

Main Methods:

  • A novel platform was designed for continuous perfusion control of μPADs.
  • The platform's components are primarily fabricated using a 3D desktop printer for accessibility.
  • The system integrates 3D printed parts with μPADs to achieve controlled fluid flow.

Main Results:

  • The developed platform successfully enables continuous perfusion in μPADs.
  • The 3D printable nature of the platform ensures low cost and easy duplication.
  • Demonstrated feasibility of μPADs for cell culturing and drug screening applications.

Conclusions:

  • The novel platform significantly enhances the capabilities of μPADs for continuous flow biofluidics.
  • The low-cost and accessible design promotes wider adoption of μPADs in research.
  • μPADs, with controlled perfusion, show promise as effective platforms for cell culturing and drug screening.