Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:23

Membrane Fluidity

168.0K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
168.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reversible control of CAR T cells through PROTAC compound targeting bromodomain mutant.

Molecular therapy. Oncology·2026
Same author

SSEL-CPP: A SHAP-based feature-selection ensemble learning framework identifies molecular properties of cell-penetrating peptides.

Protein science : a publication of the Protein Society·2026
Same author

Corrigendum to "GinDB-AI: An integrated database of Panax-derived compounds and an AI-driven platform for multidimensional information and biological activity prediction" [J Ginseng Res 50/3 (2026) 100986].

Journal of ginseng research·2026
Same author

Nano formulation of Sophora japonica extract stimulates osteogenic activity in osteoblasts.

Journal of food science and technology·2026
Same author

CONTRA-IL6: an interpretable hybrid convolutional neural network and Transformer framework for accurate prediction of interleukin-6-inducing peptides using protein language models.

Briefings in bioinformatics·2026
Same author

Integrative Peptide Drug Development: Chemical Engineering, AI-Driven Design, and Cell-Penetrating Peptides.

Pharmaceutics·2026

Related Experiment Video

Updated: Nov 21, 2025

Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release
08:39

Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release

Published on: July 4, 2017

9.2K

Decrease in membrane fluidity and traction force induced by silica-coated magnetic nanoparticles.

Tae Hwan Shin1, Abdurazak Aman Ketebo2, Da Yeon Lee1

  • 1Department of Physiology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.

Journal of Nanobiotechnology
|January 12, 2021
PubMed
Summary

Overdose of silica-coated magnetic nanoparticles [MNPs@SiO2(RITC)] impaired cell movement and biophysical properties in HEK293 cells. This highlights the need for biophysical assessments to evaluate nanoparticle side-effects.

Keywords:
Cell movementMembrane fluidityMicropillarSilica-coated magnetic nanoparticlesTraction force

More Related Videos

Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles
08:13

Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

Published on: February 27, 2021

4.8K
Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles
07:47

Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles

Published on: November 27, 2015

11.1K

Related Experiment Videos

Last Updated: Nov 21, 2025

Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release
08:39

Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release

Published on: July 4, 2017

9.2K
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles
08:13

Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

Published on: February 27, 2021

4.8K
Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles
07:47

Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles

Published on: November 27, 2015

11.1K

Area of Science:

  • Biophysics
  • Nanomedicine
  • Cell Biology

Background:

  • Nanoparticles offer unique properties for biomedical uses but pose challenges in biophysical assessment and side-effect evaluation.
  • Silica-coated magnetic nanoparticles with rhodamine B isothiocyanate [MNPs@SiO2(RITC)] were investigated for their impact on cell biophysics.

Purpose of the Study:

  • To investigate the biophysical effects of MNPs@SiO2(RITC) on human embryonic kidney 293 (HEK293) cells.
  • To analyze changes in membrane fluidity, traction force, and cell movement.
  • To explore nanoparticle-induced cellular alterations using metabolic and transcriptomic profiling.

Main Methods:

  • Treatment of HEK293 cells with varying doses of MNPs@SiO2(RITC).
  • Assessment of membrane fluidity, cell morphology, and traction forces.
  • Measurement of intracellular adenosine triphosphate (ATP) levels.
  • Metabolic profiling and transcriptomic network analysis.

Main Results:

  • High-dose MNPs@SiO2(RITC) induced lipid peroxidation, decreased membrane fluidity, and shrunk HEK293 cells.
  • Increased cellular traction force and decreased cell movement velocity were observed.
  • Intracellular ATP levels decreased dose-dependently; metabotranscriptomics revealed links between lipid peroxidation, focal adhesion, and cell movement.

Conclusions:

  • Overdose of MNPs@SiO2(RITC) negatively impacts cellular biophysical properties and movement.
  • These findings underscore the critical need for robust biophysical assessments to understand and mitigate nanoparticle-induced side-effects.