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Related Experiment Video

Updated: Nov 21, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Deciphering molecular interactions by proximity labeling.

Wei Qin¹, Kelvin F Cho^1,2, Peter E Cavanagh³

¹Department of Genetics, Stanford University, Stanford, CA, USA.

|January 12, 2021

Summary

This summary is machine-generated.

Proximity labeling (PL) tags proteins interacting with a specific

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Area of Science:

Molecular biology
Biochemistry
Cell biology

Background:

Biological processes rely on molecular interactions.
Proximity labeling (PL) tags endogenous interaction partners of specific protein 'baits'.
This technology utilizes promiscuous enzymes to generate reactive species in living cells.

Purpose of the Study:

Review the development of proximity labeling technologies.
Highlight studies applying PL for molecular interaction discovery.
Focus on mapping protein-protein, protein-RNA, and protein-DNA interactions.

Main Methods:

Genetic fusion of proteins to promiscuous enzymes.
Catalysis of diffusible reactive species generation.
Enrichment and identification of tagged molecules via mass spectrometry or sequencing.

Main Results:

PL enables the discovery of endogenous molecular interactions.
Successful mapping of protein-protein interactions.
Successful mapping of protein-RNA and protein-DNA interactions.

Conclusions:

Proximity labeling is a powerful tool for studying molecular interactions.
PL facilitates the analysis of complex biological networks in living systems.
This technology is applicable in diverse biological contexts, including whole organisms.