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Delayed Treatment with Human Dental Pulp Stem Cells Accelerates Functional Recovery and Modifies Responses of
Wai Ping Yew1, Natalia D Djukic1, Jaya S P Jayaseelan1
1Centre for Neuroscience, College of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.
Cell Transplantation
|January 12, 2021
Summary
Delayed treatment with dental pulp stem cells accelerated functional recovery after stroke in rats. This neurorestorative effect was associated with reduced glial scarring in the brain, suggesting a novel therapeutic avenue.
Area of Science:
- Neuroscience
- Regenerative Medicine
- Stem Cell Biology
Background:
- Multipotent mesenchymal stem cells from dental pulp show promise for stroke recovery.
- Previous studies focused on early intervention, but delayed treatment potential remains less explored.
Purpose of the Study:
- To investigate the efficacy of delayed human dental pulp stem cell (hDPSCs) administration after photothrombotic stroke in rats.
- To assess the impact of delayed hDPSCs on functional recovery and neuroinflammation.
Main Methods:
- Photothrombotic stroke was induced in rats, and hDPSCs or vehicle were administered 3 days post-stroke.
- Functional recovery was evaluated using forepaw placing and skilled reaching tests up to 28 days.
- Immunohistochemistry was used to assess glial fibrillary acidic protein (GFAP), synaptophysin, and collagen IV in peri-infarct tissue.
Main Results:
- hDPSCs treatment accelerated recovery in forelimb placing and initial success in skilled reaching tasks.
- No significant differences were observed in overall skilled reaching success or exploratory forepaw use.
- Reduced GFAP immunolabeling in peri-infarct tissue indicated decreased reactive astrogliosis.
Conclusions:
- Delayed administration of dental pulp stem cells can promote functional recovery after ischemic stroke.
- The neurorestorative effects may be partly mediated by reduced reactive astrogliosis in the peri-infarct area.
- hDPSCs represent a potential therapeutic strategy for stroke, even when treatment is initiated after infarct stabilization.
