Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid MIC testing with the sensititre autoreader.

J L Staneck1, S D Allen, E E Harris

  • 1Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Ohio 45267-0714.

Journal of Clinical Microbiology
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Results of the c-TRAK TN trial: a clinical trial utilising ctDNA mutation tracking to detect molecular residual disease and trigger intervention in patients with moderate- and high-risk early-stage triple-negative breast cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2022
Same author

The radiological excision of high risk and malignant lesions using the INTACT breast lesion excision system. A case series with an imaging follow up of at least 5 years.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2014
Same author

Cutaneous presentation of progressive disseminated histoplasmosis nine years after renal transplantation.

Transplant infectious disease : an official journal of the Transplantation Society·2013
Same author

Pre-operative factors indicating risk of multiple operations versus a single operation in women undergoing surgery for screen detected breast cancer.

Breast (Edinburgh, Scotland)·2012
Same author

Design and performance optimization of fiber optic adaptive filters.

Applied optics·2010
Same author

Infrared optical absorptivity and reflectivity of hot-pressed SiC.

Applied optics·2010
Same journal

Ebola laboratory preparedness at frontline hospitals: can we or can't we?

Journal of clinical microbiology·2026
Same journal

Reporting macrolide-resistant <i>Mycoplasma pneumoniae</i>: a diagnostic obligation?

Journal of clinical microbiology·2026
Same journal

Diagnostic value of HHV-6A/B genotyping in immunocompromised adults.

Journal of clinical microbiology·2026
Same journal

Multicenter performance evaluation of the Simplexa <i>C. auris</i> Direct assay for the detection of <i>Candida auris</i> colonization in bilateral axilla/groin swabs.

Journal of clinical microbiology·2026
Same journal

Comparison of blood culture contamination rates with standard practice versus two blood diversion devices at a single institution.

Journal of clinical microbiology·2026
Same journal

Risk assessment and mitigation of hepatitis C virus RNA carryover contamination in a reflex testing algorithm.

Journal of clinical microbiology·2026
See all related articles

Automated microdilution antimicrobial susceptibility testing using the Sensititre Autoreader showed high accuracy for Enterobacteriaceae and Staphylococcus aureus, comparable to manual methods but with faster results.

Area of Science:

  • Clinical Microbiology
  • Antimicrobial Susceptibility Testing
  • Laboratory Automation

Background:

  • Manual determination of Minimum Inhibitory Concentrations (MICs) is time-consuming.
  • Automated systems offer potential for faster and more efficient antimicrobial susceptibility testing.

Purpose of the Study:

  • To compare automated MIC results from the Sensititre Autoreader with manual readings.
  • To evaluate the accuracy and reproducibility of the automated system for key bacterial pathogens.

Main Methods:

  • A three-laboratory study compared Autoreader MICs (5-hour incubation) with manual MICs (18-hour incubation).
  • 704 Enterobacteriaceae and 82 Staphylococcus aureus isolates were tested against various antimicrobial agents.

Main Results:

Related Experiment Videos

  • Autoreader MICs were within 1 doubling dilution of control values in 92.9% of Enterobacteriaceae and 93.6% of S. aureus tests.
  • Discrepancies occurred more frequently with beta-lactam agents and certain species like Pseudomonas aeruginosa.
  • Methicillin-resistant S. aureus presented challenges for the automated system.

Conclusions:

  • The Sensititre Autoreader provides accurate and reproducible MIC results for Enterobacteriaceae and S. aureus.
  • The automated system maintains the advantages of broth microdilution while offering faster turnaround times.