Microbial Perchlorate Reduction Driven by Ethane and Propane

Previous studies demonstrated that methane can be used as an electron donor to microbially remove various oxidized contaminants in groundwater. Natural gas, which is more widely available and less expensive than purified methane, is potentially an alternative source of methane. However, natural gas commonly contains a considerable amount of ethane (C₂H₆) and propane (C₃H₈), in addition to methane. It is important that these gaseous alkanes are also utilized along with methane to avoid emissions. Here, we demonstrate that perchlorate (ClO₄^-), a frequently reported contaminant in groundwater, can be microbially reduced to chloride (Cl^-) driven by C₂H₆ or C₃H₈ under oxygen-limiting conditions. Two independent membrane biofilm reactors (MBfRs) supplied with C₂H₆ and C₃H₈, respectively, were operated in parallel to biologically reduce ClO₄^-. The continuous ClO₄^- removal during long-term MBfR operation combined with the concurrent C₂H₆/C₃H₈ consumption and ClO₄^- reduction in batch tests confirms that ClO₄^- reduction was associated with C₂H₆ or C₃H₈ oxidation. Polyhydroxyalkanoates (PHAs) were synthesized in the presence of C₂H₆ or C₃H₈ and were subsequently utilized for supporting ClO₄^- bio-reduction in the absence of gaseous alkanes. Analysis by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) showed that transcript abundance of bmoX (encoding alpha hydroxylase subunit of C₂H₆/C₃H₈ monooxygenase) was positively correlated to the consumption rates of C₂H₆/C₃H₈, while pcrA (encoding a catalytic subunit of perchlorate reductase) was positively correlated to the consumption of ClO₄^-. High-throughput sequencing targeting 16S rRNA, bmoX, and pcrA indicated that Mycobacterium was the dominant microorganism oxidizing C₂H₆/C₃H₈, while Dechloromonas may be the major perchlorate-reducing bacterium in the biofilms. These findings shed light on microbial ClO₄^- reduction driven by C₂H₆ and C₃H₈, facilitating the development of cost-effective strategies for ex situ groundwater remediation.