Capturing Circulating Tumor Cells through a Combination of Hierarchical Nanotopography and Surface Chemistry

Circulating tumor cells (CTCs) have become known as minimally invasive multifunctional biomarkers for earlier diagnosis, prognosis, recurrence risk assessment, and therapeutic monitoring in recent years. However, effectively capturing these CTCs is still difficult because of the extremely low abundance of CTCs and the diverse phenotypes of cancer cells. In this study, we present a novel necklace-like polydopamine nanosphere (PDA NS)/alginate composite nanofiber with a hierarchical nanotopographical structure and a surface chemical signal for capturing the CTCs. The height of the nanotopography, which is formed by connecting PDA NSs with nanofibers via electrospinning, can be easily adjusted by changing the size of the PDA NSs. Four types of cancer cells are employed to investigate the capture efficiency of the fiber. More importantly, in a blood environment containing rare cancer cells, the fiber still has a great ability to capture these cells. Therefore, this nanofiber is identified as a potential device for the diagnosis of cancer.