Related Experiment Video
Updated: Nov 21, 2025
Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections
Published on: February 2, 2024
circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty
Shenghui Ni1,2, Tianlong Jiang1, Shimin Hao1
1Department of Orthopedic Surgery, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, 110001, P.R. China.
Insights
Circular RNAs (circRNAs) play a role in aseptic loosening after hip replacement. This study identified key circRNAs and their regulatory networks, revealing potential new treatment strategies.
Area of Science:
- Biochemistry
- Molecular Biology
- Orthopedics
Background:
- Circular RNAs (circRNAs) are increasingly recognized for their roles in disease pathogenesis.
- The involvement of circRNAs in aseptic loosening following total hip arthroplasty (THA) is not well understood.
Purpose of the Study:
- To identify differentially expressed circRNAs (DEcircRNAs) in aseptic loosening after THA.
- To elucidate the regulatory mechanisms of circRNAs in this condition.
Main Methods:
- RNA sequencing (RNA-seq) to identify DEcircRNAs.
- Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for validation.
- Competing endogenous RNA (ceRNA) analysis to predict circRNA-miRNA-mRNA networks.
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
Main Results:
- 257 DEcircRNAs were identified via RNA-seq.
- Six specific circRNAs were validated using RT-qPCR.
- A circRNA-miRNA-mRNA network involving 6 circRNAs, 138 miRNAs, and 1667 mRNAs was constructed.
- Pathway analysis indicated circRNAs are linked to aseptic loosening-related biological functions and pathways.
Conclusions:
- The study established a circRNA-miRNA-mRNA network relevant to aseptic loosening after THA.
- Findings suggest circRNAs may offer novel insights into the pathogenesis and potential therapeutic targets for aseptic loosening.
Abstract:
Increasing evidence has demonstrated that circular RNA (circRNA) exerts important function in the pathogenesis of some diseases. While, the contributions of circRNAs to aseptic loosening after total hip arthroplasty (THA) remain largely unknown. Our research is to explore the differentially expressed circRNAs (DEcircRNAs) and elucidate complex regulated mechanism of circRNAs in aseptic loosening. The DEcircRNAs were identified by RNA sequencing (RNA-seq) analysis. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was adopted to corroborate these DEcircRNAs. The potential function of circRNAs in aseptic loosening tissue was identified by competing endogenous RNA (ceRNA) analysis. Enrichment analysis was performed for target mRNAs and host genes of the DEcircRNAs by Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). 257 DEcircRNAs were obtained from RNA-seq results. Following the RT-qPCR corroboration, 6 circRNAs (hsa_circ_0007482, hsa_circ_0005232, hsa_circ_0000994, hsa_circ_0000690, hsa_circ_0058092 and hsa_circ_0004496) were selected for further analysis. By circRNA-miRNA and miRNA-mRNA prediction, 6 circRNAs, 138 miRNAs and 1667 mRNAs were identified. Then, circRNA-miRNA-mRNA network was established. The result of GO and KEGG enrichment analysis suggested that the circRNAs were related with some biological functions and pathways of aseptic loosening. A novel pathogenesis and treatment strategy about aseptic loosening after THA was revealed from our study of circRNA-miRNA-mRNA network.
