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Related Experiment Video

Updated: Nov 21, 2025

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites
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Actin-depolymerizing factor from Eimeria tenella promotes immunogenic function of chicken dendritic cells.

Shakeel Ahmed Lakho1, Muhammad Haseeb1, Jianmei Huang1

  • 1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.

Parasitology Research
|January 13, 2021
PubMed
Summary
This summary is machine-generated.

The actin-depolymerizing factor from Eimeria tenella (EtADF) activates chicken dendritic cells (ChDCs), promoting T cell proliferation and Th1 immune responses. This study offers insights for developing anticoccidial vaccines against Eimeria tenella.

Keywords:
Actin-depolymerizing factorAvian Eimeria speciesCD4+ T cellsCytokinesDendritic cellsToll-like receptors

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Area of Science:

  • Immunology
  • Parasitology
  • Veterinary Science

Background:

  • Dendritic cells (DCs) are vital for initiating antigen-specific immunity against pathogens.
  • Understanding host immune responses to parasite molecules is key for developing control strategies.

Purpose of the Study:

  • To evaluate the capacity of chicken dendritic cells (ChDCs) to respond to actin-depolymerizing factor from Eimeria tenella (EtADF).
  • To assess EtADF's role in T cell proliferation and differentiation for potential anticoccidial vaccine development.

Main Methods:

  • Recombinant EtADF (rEtADF) was used to treat ChDCs derived from chicken spleens.
  • Immunoblot, immunofluorescence, flow cytometry, and qRT-PCR were employed to analyze ChDC activation and signaling.
  • DC/T cell co-incubation systems were used to assess T cell responses.

Main Results:

  • rEtADF bound to ChDCs, increasing surface markers (MHCII, CD1.1, CD11c, CD80, CD86) and triggering TLR signaling while suppressing Wnt signaling.
  • rEtADF enhanced ChDC expression of CD83, CCL5, and CCR7, promoting T cell proliferation and differentiation into CD3+/CD4+ T cells.
  • rEtADF-pulsed ChDCs increased IL-12 and IFN-γ production, facilitating Th1 polarization and enhancing IFN-γ secretion in co-cultured T cells.

Conclusions:

  • EtADF activates chicken dendritic cells and promotes Th1-biased immune responses.
  • EtADF shows potential as a component in anticoccidial vaccine strategies.