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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Protein Glycosylation01:25

Protein Glycosylation

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Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
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Related Experiment Video

Updated: Nov 21, 2025

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
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Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

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Towards structure-focused glycoproteomics.

Anastasia Chernykh1, Rebeca Kawahara1, Morten Thaysen-Andersen1,2

  • 1Department of Molecular Sciences, Macquarie University, Sydney, NSW, Australia.

Biochemical Society Transactions
|January 13, 2021
PubMed
Summary
This summary is machine-generated.

Glycoproteomics advances enable detailed analysis of protein glycosylation. Structure-focused glycoproteomics is emerging, revealing intricate glycan structures and their sites on proteins for systems glycobiology insights.

Keywords:
LC-MS/MSglycopeptideglycoproteinsglycoproteomicsglycosylationmass spectrometry

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Area of Science:

  • Glycosciences
  • Proteomics
  • Mass Spectrometry
  • Bioinformatics

Background:

  • Glycoproteomics, the study of intact glycopeptides, has matured due to advances in separation sciences, mass spectrometry, and informatics.
  • Quantitative glycoproteomics strategies are available for mapping glycan compositions to protein sites, but structure-focused analysis remains a frontier.

Purpose of the Study:

  • To review recent glycoproteomics studies (2018-2020) and summarize technological innovations in structure-focused glycoproteomics.
  • To highlight the growing importance of understanding glycan fine structures at specific protein sites.

Main Methods:

  • Survey of glycoproteomics studies published between 2018 and 2020.
  • Summarization of technological advancements in structure-focused glycoproteomics.
  • Analysis of emerging chemoenzymatic labeling, enrichment, separation, and mass spectrometry detection methods.

Main Results:

  • Quantitative glycoproteomics is increasingly used for N-glycoproteome profiling, while the O-glycoproteome remains less explored.
  • New technologies are rapidly emerging for structure-focused glycoproteomics, enabling detailed analysis of glycan fine structures.
  • Site-specific glycan details like bisecting GlcNAcylation, fucosylation, and sialyl-linkage are being revealed.

Conclusions:

  • Glycoproteomics is a key component of glycosciences, expanding its reach.
  • Structure-focused glycoproteomics holds significant potential for advancing systems glycobiology and understanding the glycoproteome.
  • Emerging technologies promise to unlock deeper insights into glycan structures and their biological roles.