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Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
Published on: October 31, 2012
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Generation and manipulation of human iPSC-derived platelets
Naoshi Sugimoto1, Koji Eto2,3
1Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan. sugimoto.naoshi.4v@kyoto-u.jp.
Cellular and Molecular Life Sciences : CMLS
|January 13, 2021
Summary
Human induced pluripotent stem cells (iPSCs) are being developed into platelets for transfusion, offering a safe, donor-independent alternative. This innovation aims to enhance transfusion medicine and overcome platelet transfusion refractoriness.
Area of Science:
- Regenerative Medicine
- Hematology
- Cell Therapy
Background:
- Induced pluripotent stem cells (iPSCs) offer a promising source for generating various cell types for therapeutic applications.
- Current platelet transfusion relies on donor blood, facing limitations like pathogen transmission risks and alloimmune refractoriness.
- iPSC-derived platelets present a potential solution for donor-independent, pathogen-free, and personalized transfusion products.
Purpose of the Study:
- To detail the advancements enabling the large-scale production of functional platelets from human iPSCs.
- To highlight the potential of iPSC-derived platelets to revolutionize transfusion medicine and address current supply challenges.
- To underscore the safety and efficacy considerations for clinical translation of iPSC-derived platelets.
Main Methods:
- Establishment of expandable megakaryocytes from iPSCs using transgene expression.
- Development of a turbulent-type bioreactor for enhanced platelet yield and quality.
- Implementation of feeder cell-free culture conditions using novel drugs.
- Purification and resuspension of platelets in a specialized buffer.
- Preclinical safety and competency assessments, including non-tumorigenicity testing.
Main Results:
- Successful generation of expandable megakaryocytes from iPSCs.
- Optimized bioreactor and culture conditions leading to improved platelet production.
- Demonstrated safety and competency of iPSC-derived platelets through in vitro and in vivo studies.
- Initiation of a clinical trial based on these advancements.
Conclusions:
- The generation of human iPSC-derived platelets represents a significant advancement in transfusion medicine.
- This technology promises a ubiquitous, safe, and donor-independent supply of platelet products.
- Future gene manipulation in iPSCs could lead to novel therapeutic platelet products.
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