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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Microtubules in Signaling01:22

Microtubules in Signaling

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The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
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Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

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The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
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Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

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The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
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Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Related Experiment Video

Updated: Nov 21, 2025

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
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Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors

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NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression.

Ji Pang1, Liang Le2, Yi Zhou3

  • 1Stowers Institute for Medical Research, 1000 East 50(th) Street, Kansas City, MO 64110, USA; School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.

Cell Reports
|January 13, 2021
PubMed
Summary

NOTCH signaling in the ciliary body (CB) is crucial for maintaining eye structures and intraocular pressure (IOP). This pathway regulates CB development, aqueous humor secretion, and vitreous protein levels, offering insights into eye diseases.

Keywords:
Cx43IOPNOTCH signalingNectin1anterior segmentaqueous humorciliary bodyeye degenerationmorphogenesissecretionviteous

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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Last Updated: Nov 21, 2025

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

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Area of Science:

  • Ophthalmology
  • Developmental Biology
  • Cell Signaling

Background:

  • Anterior segment dysgenesis is linked to cornea diseases, cataracts, and glaucoma.
  • The ciliary body (CB) secretes aqueous humor, maintaining intraocular pressure (IOP), but its development and function are poorly understood.

Purpose of the Study:

  • To elucidate the role of NOTCH signaling in the ciliary body (CB) for eye development and function.
  • To understand how NOTCH signaling regulates intraocular pressure (IOP) and vitreous humor composition.

Main Methods:

  • Investigated NOTCH signaling pathways (Notch2, Notch3, RBPJ) in the CB.
  • Analyzed effects on ciliary epithelia adhesion, CB morphogenesis, and aqueous humor secretion.
  • Examined regulation of Nectin1 and Cx43 expression and vitreous protein secretion.

Main Results:

  • NOTCH signaling in the CB maintains vitreous integrity, IOP, and overall eye structure.
  • Notch2 and Notch3 signaling via RBPJ controls CB morphogenesis and aqueous humor secretion.
  • NOTCH signaling regulates Nectin1 and Cx43 to promote cell adhesion and control secretion, and directly impacts vitreous protein secretion.

Conclusions:

  • NOTCH signaling is essential for CB development and function, maintaining IOP and eye structure.
  • This study provides critical insights into the mechanisms underlying CB function and its involvement in ocular diseases.