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NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression
1Stowers Institute for Medical Research, 1000 East 50(th) Street, Kansas City, MO 64110, USA; School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
Cell Reports
|January 13, 2021
Summary
NOTCH signaling in the ciliary body (CB) is crucial for maintaining eye structures and intraocular pressure (IOP). This pathway regulates CB development, aqueous humor secretion, and vitreous protein levels, offering insights into eye diseases.
Area of Science:
- Ophthalmology
- Developmental Biology
- Cell Signaling
Background:
- Anterior segment dysgenesis is linked to cornea diseases, cataracts, and glaucoma.
- The ciliary body (CB) secretes aqueous humor, maintaining intraocular pressure (IOP), but its development and function are poorly understood.
Purpose of the Study:
- To elucidate the role of NOTCH signaling in the ciliary body (CB) for eye development and function.
- To understand how NOTCH signaling regulates intraocular pressure (IOP) and vitreous humor composition.
Main Methods:
- Investigated NOTCH signaling pathways (Notch2, Notch3, RBPJ) in the CB.
- Analyzed effects on ciliary epithelia adhesion, CB morphogenesis, and aqueous humor secretion.
- Examined regulation of Nectin1 and Cx43 expression and vitreous protein secretion.
Main Results:
- NOTCH signaling in the CB maintains vitreous integrity, IOP, and overall eye structure.
- Notch2 and Notch3 signaling via RBPJ controls CB morphogenesis and aqueous humor secretion.
- NOTCH signaling regulates Nectin1 and Cx43 to promote cell adhesion and control secretion, and directly impacts vitreous protein secretion.
Conclusions:
- NOTCH signaling is essential for CB development and function, maintaining IOP and eye structure.
- This study provides critical insights into the mechanisms underlying CB function and its involvement in ocular diseases.
Keywords:
Cx43IOPNOTCH signalingNectin1anterior segmentaqueous humorciliary bodyeye degenerationmorphogenesissecretionviteousMore Related Videos
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