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A novel borate fluorescent probe for rapid selective intracellular peroxynitrite imaging
Minmin Wang1, Chun Wang2, Wenwu Song3
1School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, PR China; Nantong Key Laboratory of Intelligent and New Energy Materials, PR China.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|January 13, 2021
Summary
Researchers developed a novel fluorescent probe to detect peroxynitrite (ONOO-), a key reactive nitrogen species. This sensitive probe successfully monitored ONOO- levels in both cancer and normal cells.
Area of Science:
- Biochemistry
- Chemical Biology
- Molecular Imaging
Background:
- Reactive nitrogen species (RNS), including peroxynitrite (ONOO-), are crucial in biological processes like antibacterial activity and signal transduction.
- Dysregulation of ONOO- is implicated in various physiological and pathological conditions, necessitating accurate detection methods.
Purpose of the Study:
- To design and synthesize a novel, highly selective, and sensitive fluorescent probe for ONOO- detection.
- To investigate the mechanism of interaction between the designed probe and ONOO-.
- To evaluate the probe's performance in biological samples, including monitoring ONOO- levels in cells.
Main Methods:
- Design and synthesis of a fluorescent probe incorporating a benzyl borate group.
- Spectroscopic analysis to determine the probe's response to ONOO- across a wide pH range.
- Cell permeability studies and fluorescence imaging to monitor intracellular ONOO- levels in cancer and normal cells.
Main Results:
- The developed fluorescent probe exhibits high selectivity and sensitivity towards ONOO-.
- The probe demonstrates a strong fluorescence response to ONOO- in a broad pH range (7.0-11.5).
- The probe shows good cell permeability, enabling successful monitoring of ONOO- content in both cancer and normal cells.
Conclusions:
- A novel benzyl borate-based fluorescent probe for selective and sensitive ONOO- detection has been successfully developed.
- The probe's robust performance across physiological pH and its cell permeability make it a valuable tool for biological research.
- This fluorescent probe facilitates the monitoring of ONOO- levels in cellular environments, aiding in understanding its role in health and disease.
