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Updated: Nov 21, 2025

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Quantitative Mass Spectrometry Imaging of Biological Systems
Daisy Unsihuay1, Daniela Mesa Sanchez1, Julia Laskin1
1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA; email: dunsihua@purdue.edu, dmesasan@purdue.edu, jlaskin@purdue.edu.
Abstract:
Mass spectrometry imaging (MSI) is a powerful, label-free technique that provides detailed maps of hundreds of molecules in complex samples with high sensitivity and subcellular spatial resolution. Accurate quantification in MSI relies on a detailed understanding of matrix effects associated with the ionization process along with evaluation of the extraction efficiency and mass-dependent ion losses occurring in the analysis step. We present a critical summary of approaches developed for quantitative MSI of metabolites, lipids, and proteins in biological tissues and discuss their current and future applications.
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