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Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice
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Short-term fasting reshapes fat tissue.

Tuohua Mao1, Quanwei Wei2,3, Fang Zhao2

  • 1Department of Endocrinology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.

Endocrine Journal
|January 14, 2021
PubMed
Summary
This summary is machine-generated.

Acute fasting (AF) reshapes fat tissue, improving lipid metabolism and promoting "browning" of white adipose tissue. These beneficial effects may involve Sirt5 regulation and altered succinylation in fatty tissues.

Keywords:
Acute fasting (AF)Adipose-tissue remodelingBrowning of white fatMacrophage M2Sirt5

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Area of Science:

  • Metabolic research
  • Obesity and adipose tissue biology

Background:

  • Intermittent fasting is a growing area of research for obesity and metabolic syndrome.
  • Adipose tissue plays a key role in metabolism and obesity development.

Purpose of the Study:

  • To investigate the effects of acute fasting (AF) on adipose tissue.
  • To explore the underlying mechanisms of AF's impact on fat metabolism.

Main Methods:

  • Mice were subjected to 36-hour acute fasting on either a low-fat diet (LFD) or high-fat diet (HFD).
  • Control groups were fed without fasting.
  • Changes in adipose tissue morphology, macrophage polarization, Sirt5 expression, and succinylation were analyzed.

Main Results:

  • AF significantly altered white adipose tissue (WAT) morphology and promoted WAT beiging in both LFD and HFD mice.
  • AF improved lipid metabolism and increased M2 macrophage polarization in HFD-fed mice.
  • AF upregulated Sirt5 expression and increased fat tissue succinylation.

Conclusions:

  • Acute fasting remodels adipose tissue, offering beneficial effects on fat metabolism.
  • AF-induced improvements may be mediated by Sirt5 regulation and altered succinylation.
  • This study provides new insights into energy restriction's impact on fat metabolism via succinylation.