Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.1K
CRISPR01:59

CRISPR

55.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
55.1K
Homologous Recombination02:31

Homologous Recombination

59.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
59.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.4K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NMR of Fully and Partially <sup>13</sup>C-Enriched Biomass Enhances Pendent Group Structural Characterization.

Analytical chemistry·2026
Same author

The role of phages in plant-associated microbial communities.

Essays in biochemistry·2026
Same author

Author Correction: Membrane remodelling mediates lipopeptide-induced immunity in Arabidopsis.

Nature plants·2026
Same author

Nuclear SnRK1 Activity Delays Clubroot Development in Arabidopsis by Reducing Sink Strength.

Plant, cell & environment·2026
Same author

Membrane remodelling mediates lipopeptide-induced immunity in Arabidopsis.

Nature plants·2026
Same author

Cell-specific transcriptomics and knockout reveal aquaporin function in grass stomatal movements.

The New phytologist·2026

Related Experiment Video

Updated: Nov 21, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.9K

Efficient CRISPR-mediated base editing in Agrobacterium spp.

Savio D Rodrigues1, Mansour Karimi2,3, Lennert Impens2,3

  • 1Division of Crop Biotechnics, Department of Biosystems, KU Leuven, 3001 Leuven, Belgium.

Proceedings of the National Academy of Sciences of the United States of America
|January 14, 2021
PubMed
Summary

We developed CRISPR-mediated base editing for efficient Agrobacterium genome engineering. This method allows targeted mutations for improved plant transformation and gene editing applications.

Keywords:
AgrobacteriumCRISPRbase editinghairy root diseaseplant transformation

More Related Videos

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.2K
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.8K

Related Experiment Videos

Last Updated: Nov 21, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.9K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.2K
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.8K

Area of Science:

  • Microbiology
  • Plant Biotechnology
  • Genetic Engineering

Background:

  • Agrobacterium spp. are crucial for plant genetic engineering but traditional mutation strategies are slow.
  • Existing Agrobacterium laboratory strains have seen limited updates for decades.
  • Functional gene analysis in Agrobacterium is hindered by laborious mutation methods.

Purpose of the Study:

  • To develop an efficient method for targeted point mutations in Agrobacterium genomes.
  • To enable rapid functional gene analysis and strain improvement in Agrobacterium.
  • To advance plant genetic engineering tools by enhancing Agrobacterium capabilities.

Main Methods:

  • Utilized clustered regularly interspaced short palindromic repeats (CRISPR)-mediated base editing.
  • Applied the technique to Agrobacterium tumefaciens and Agrobacterium rhizogenes.
  • Generated loss-of-function recA mutations in EHA105 and analyzed RolB/RolC genes in K599.

Main Results:

  • Achieved high editing efficiency (80% of cells) in 90% of transformed colonies.
  • Successfully generated functional recA mutant strains for maize transformation.
  • Confirmed the role of RolB and RolC in hairy root development.
  • Identified Cas9-independent off-target mutations, highlighting TC motifs as hotspots.

Conclusions:

  • CRISPR-mediated base editing offers an efficient approach for Agrobacterium genome engineering.
  • This technology facilitates rapid functional analysis and the development of improved strains.
  • The findings pave the way for 'engineering the engineer' for enhanced plant biotechnology.