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Related Experiment Video

Updated: Nov 21, 2025

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets
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Liquid Phase Peptide Synthesis via One-Pot Nanostar Sieving (PEPSTAR).

Jet Yeo1, Ludmila Peeva1, Seoyeon Chung1

  • 1Barrer Centre, Department of Chemical Engineering, Imperial College London, Exhibition Road, London, SW7 2AZ, UK.

Angewandte Chemie (International Ed. in English)
|January 14, 2021
PubMed
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This study introduces a novel one-pot liquid phase peptide synthesis method using a nanostar support and organic solvent nanofiltration (OSN). This PEPSTAR approach enhances efficiency and purity for peptide synthesis.

Area of Science:

  • Chemical Synthesis
  • Biotechnology
  • Materials Science

Background:

  • Liquid phase peptide synthesis (LPPS) traditionally involves complex purification steps.
  • Solid phase peptide synthesis (SPPS) can generate significant waste and requires specialized resins.
  • There is a need for more efficient and sustainable peptide synthesis methodologies.

Purpose of the Study:

  • To develop a one-pot liquid phase peptide synthesis method.
  • To integrate organic solvent nanofiltration (OSN) for in-situ purification.
  • To utilize a novel nanostar support for improved synthesis and monitoring.

Main Methods:

  • Iterative addition of amino acids to a nanostar support in a liquid phase.
  • Employing organic solvent nanofiltration (OSN) for isolating the growing peptide after each cycle.
Keywords:
liquid phase peptide synthesismembranesorganic solvent nanofiltration (OSN)peptides

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  • Utilizing a reactor-separator apparatus for seamless coupling, Fmoc removal, and byproduct filtration.

    • Main Results:

    • Successfully synthesized enkephalin-like peptides, octreotate amide, and octreotate with high crude purity.
    • Demonstrated efficient nanofiltration and real-time reaction monitoring facilitated by the nanostar support.
    • Achieved comparable or superior purity to vendor-produced solid-phase synthesis samples.

    Conclusions:

    • The PEPSTAR method offers an efficient and streamlined approach to peptide synthesis.
    • Integration of OSN with a nanostar support overcomes limitations of traditional LPPS and SPPS.
    • This novel technique provides a sustainable and effective alternative for producing peptides.