Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-linked Receptors01:00

Enzyme-linked Receptors

83.1K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
83.1K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.3K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.3K
Neural Regulation01:37

Neural Regulation

41.9K
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
41.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of the genotypic and phenotypic spectrum of TCF7L2-related neurodevelopmental disorder (TRND).

Genetics in medicine : official journal of the American College of Medical Genetics·2026
Same author

Sheep erythrocyte potassium locus highlights MYADM family regulation of electroneutral potassium chloride cotransporters (KCC).

BMC genomics·2026
Same author

Corneal Innervation Research at a Crossroads: A Tool-Driven Roadmap for the Future.

Investigative ophthalmology & visual science·2026
Same author

Autoinflammatory syndromes of STING and TREX1 dysfunction.

The Journal of clinical investigation·2026
Same author

Changes in interstitial cells of Cajal calcium activity in a diabetic mouse model of delayed gastric emptying.

The Journal of physiology·2026
Same author

Factors to Consider in Gut Microbiota Interactions With the Enteric Nervous System-What You Look for Is What You Get.

Cellular and molecular gastroenterology and hepatology·2026

Related Experiment Video

Updated: Nov 21, 2025

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
08:26

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

Published on: September 18, 2013

9.5K

scRNA-Seq Reveals New Enteric Nervous System Roles for GDNF, NRTN, and TBX3.

Christina M Wright1, Sabine Schneider1, Kristen M Smith-Edwards2

  • 1Department of Pediatrics, Abramson Research Center, Children's Hospital of Philadelphia Research Institute, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania.

Cellular and Molecular Gastroenterology and Hepatology
|January 14, 2021
PubMed
Summary

This study identified specific genes in enteric nervous system (ENS) neuron subtypes, revealing new therapeutic targets for bowel motility disorders and enhancing understanding of ENS function.

Keywords:
Calcium ImagingHuman and Mouse ColonPou3f3 (Brn1)Transcription Factors

More Related Videos

Immunostaining to Visualize Murine Enteric Nervous System Development
07:54

Immunostaining to Visualize Murine Enteric Nervous System Development

Published on: April 29, 2015

11.7K
Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
07:53

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

Published on: August 15, 2018

13.9K

Related Experiment Videos

Last Updated: Nov 21, 2025

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
08:26

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

Published on: September 18, 2013

9.5K
Immunostaining to Visualize Murine Enteric Nervous System Development
07:54

Immunostaining to Visualize Murine Enteric Nervous System Development

Published on: April 29, 2015

11.7K
Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
07:53

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

Published on: August 15, 2018

13.9K

Area of Science:

  • Neuroscience
  • Gastroenterology
  • Molecular Biology

Background:

  • Bowel function relies on complex enteric nervous system (ENS) activity.
  • Enteric neuropathies significantly impair gastrointestinal motility.
  • Understanding ENS neuron subtypes is crucial for therapeutic development.

Purpose of the Study:

  • To define gene expression profiles in distinct enteric neuron subtypes.
  • To identify novel molecular targets for treating enteric neuropathies.
  • To elucidate the functional roles of specific genes in ENS regulation.

Main Methods:

  • Single-nucleus and single-cell RNA sequencing of mouse and human colon myenteric plexus.
  • Immunohistochemistry and mutant mouse models for gene validation.
  • Calcium imaging to assess neuronal activity and smooth muscle response.

Main Results:

  • Characterized seven adult and eight embryonic mouse ENS neuron subtypes with hundreds of differentially expressed genes.
  • Identified differential expression of BNC2, PBX3, SATB1, RBFOX1, TBX2, and TBX3 in human and mouse myenteric neurons.
  • Demonstrated that Tbx3 regulates NOS1-expressing neurons and GDNF/neurturin differentially modulate neuronal activity and smooth muscle contractions.

Conclusions:

  • Single-cell analysis elucidated subtype-specific genes in myenteric neurons.
  • Identified novel functions for TBX3, GDNF, and NRTN in ENS regulation.
  • Provided data for molecular diagnostics and therapeutics for bowel motility disorders.