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Published on: March 14, 2025
Novel method to obtain human non-separated histological samples for the assessment of peri-implant soft tissue
Dominik Kraus1, Werner Götz2, Stefan Bayer1
1Department of Prosthodontics, Preclinical Education and Material Sciences, University of Bonn, Bonn, Germany.
Objectives:
To introduce a standardized and less invasive clinical model that provides histological information on the abutment-mucosa interface in humans.
Materials And Methods:
New experimental healing abutments were left in an open healing position on bone-level implants in the interforaminal region of the mandibles in six edentulous patients. The one-piece abutments were hollow cylinder-shaped with two lateral openings that allow for ingrowth of the peri-implant mucosa into the central abutment cavity. After three months of healing, abutments and ingrown mucosa were sampled and processed for histological analysis in a non-separated resin-embedding technique. To test the validity of the new model, the ingrown tissue was compared to the peri-implant mucosa around the same samples.
Results:
None of the experimental abutments exhibited signs of failure, and all samples showed mucosal ingrowth to the inner-abutment cavity. Comparison of ingrown tissue and peri-implant mucosa revealed no significant differences regarding the traits: tissue morphology, quality of collagen fibers, and adherence to the abutment. Ingrown mucosa exhibited a tendency for higher leukocyte infiltration.
Conclusions:
The presented model is a promising approach to reduce invasiveness during the sampling process for human non-separated abutment biopsies.
