Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Nov 21, 2025

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Published on: June 22, 2012

Improved Antithrombotic Function of Oriented Endothelial Cell Monolayer on Microgrooves.

Jia-Yan Chen¹, Mi Hu¹, He Zhang¹

¹MOE Key Laboratory of Macromolecule Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.

ACS Biomaterials Science & Engineering

|January 15, 2021

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparison of prognostic outcomes in vitrectomy for myopic foveoschisis with foveal detachment: air tamponade versus no-air tamponade.

International journal of retina and vitreous·2026

Same author

Gut-Liver Axis Mechanisms Underlying Spontaneous Reversal of Liver Fibrosis: A Gut Microbiota-Metabolomics Analysis.

Metabolites·2026

Same author

Non-invasive monitoring of hyperuricemia via a flexible SERS sensor with ultrahigh sensitivity.

Mikrochimica acta·2026

Same author

EGCG inactivates tumor necrosis factor-alpha (TNFα) by inducing its higher-order assembly.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026

Same author

Cas9-PALB2 fusion protein enhances CRISPR/Cas9 mediated gene knock-in efficiency.

Journal of bioscience and bioengineering·2026

Same author

Implantable core-shell microneedles enable biphasic release of insulin for immediate and sustained glycemic regulation.

International journal of biological macromolecules·2026

Orienting endothelial cells (ECs) on microgrooved surfaces enhances their antithrombotic properties. This study reveals size-dependent EC responses to topography, improving vascular implant safety.

Area of Science:

Biomaterials Science
Cell Biology
Vascular Biology

Background:

Endothelial cell (EC) monolayer formation is crucial for vascular implant safety and function.
Oriented EC monolayers may improve endothelial properties like athero-resistance, but their antithrombotic effects are less understood.

Purpose of the Study:

To investigate the impact of microgrooved polydimethylsiloxane substrates on EC orientation and behavior.
To evaluate the antithrombotic function of oriented EC monolayers compared to those on flat surfaces.

Main Methods:

Utilized microgrooved polydimethylsiloxane substrates to guide EC orientation.
Assessed EC responses to varying groove sizes, including cytoskeleton and nuclear alignment, migration, and velocity.
Evaluated antithrombotic capabilities through gene expression, prostacyclin and tissue plasminogen activator production, and activated coagulation time.

Keywords:

antithrombotic function behaviors microgrooved polydimethylsiloxane oriented endothelial cell monolayer

More Related Videos

Quantitation of Endothelial Cell Adhesiveness In Vitro

Quantitation of Endothelial Cell Adhesiveness In Vitro

Published on: June 18, 2015

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Published on: August 30, 2007

Related Experiment Videos

Last Updated: Nov 21, 2025

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Published on: June 22, 2012

Quantitation of Endothelial Cell Adhesiveness In Vitro

Quantitation of Endothelial Cell Adhesiveness In Vitro

Published on: June 18, 2015

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Published on: August 30, 2007

Main Results:

ECs exhibited size-dependent responses to microgrooves, showing enhanced orientation, migration, and velocity.
Oriented EC monolayers on grooved substrates demonstrated superior antithrombotic properties compared to flat surfaces.
Improved antithrombotic function, including gene expression and mediator production, was particularly pronounced with smaller groove sizes.

Conclusions:

Microgrooved topography effectively guides EC orientation and influences their behavior.
Oriented EC monolayers possess enhanced antithrombotic capabilities, offering potential for improved vascular implant design.
Optimizing groove dimensions is key to maximizing the antithrombotic benefits of EC monolayers on implants.