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Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
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Thermosensitive Polymer Biocompatibility Based on Interfacial Structure at Biointerface.

Daiki Murakami, Yoko Kitahara, Shingo Kobayashi1

  • 1Frontier Center for Organic System Innovations, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan.

ACS Biomaterials Science & Engineering
|January 15, 2021
PubMed
Summary

The biocompatible polymer PMe2MA forms reversible nanoprotrusions above its lower critical solution temperature. These structures promote protein and cell adhesion, suggesting they act as scaffolds for biological interactions.

Keywords:
atomic force microscopybiocompatible polymerinterfaceintermediate water

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Area of Science:

  • Materials Science
  • Biomaterials Science
  • Polymer Science

Background:

  • Thermosensitive polymers offer tunable properties for biomedical applications.
  • Understanding polymer-water interfaces is crucial for controlling biological interactions.
  • Poly[2-(2-methoxyethoxy)ethyl methacrylate] (PMe2MA) is a biocompatible thermosensitive polymer.

Purpose of the Study:

  • To investigate the interfacial structure of PMe2MA in phosphate-buffered saline (PBS) at different temperatures.
  • To correlate structural changes with biological interactions like protein adsorption and cell adhesion.
  • To elucidate the role of nanostructures in mediating these interactions.

Main Methods:

  • Atomic force microscopy (AFM) was used to visualize the polymer-water interface.
  • Temperature-dependent studies were conducted across the polymer's phase transition.
  • Protein adsorption and platelet adhesion assays were performed.
  • NIH3T3 cell adhesion and detachment experiments were conducted.

Main Results:

  • Reversible nanometer-scale protrusions formed on the PMe2MA interface above its lower critical solution temperature (19 °C) at 37 °C, disappearing at 22 °C.
  • These structural changes were attributed to microphase separation of the polymer and water.
  • Protein adsorption and platelet adhesion were significantly reduced at 22 °C compared to 37 °C.
  • Cell detachment from the PMe2MA surface correlated with the dissipation of protrusions.

Conclusions:

  • The nanoprotrusions formed by PMe2MA act as scaffolds for protein and cell adhesion.
  • Controlling the interfacial structure of thermosensitive polymers can modulate biological interactions.
  • PMe2MA's reversible structural changes offer potential for tunable biomaterial interfaces.