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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

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Published on: July 4, 2011

Intracellular Ionic Strength Sensing Using NanoLuc.

Tausif Altamash¹, Wesam Ahmed¹, Saad Rasool¹

¹College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation, Doha 34110, Qatar.

International Journal of Molecular Sciences

|January 15, 2021

Summary

This summary is machine-generated.

Researchers developed a novel bioluminescence-based tool using Nano Luciferase (NanoLuc) to monitor intracellular ionic strength in real-time. This new biosensor is crucial for understanding cellular processes and disease progression.

Keywords:

NanoLuc bioluminescence biosensor ionic strength

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Area of Science:

Biochemistry
Cell Biology
Biophysics

Background:

Intracellular ionic strength is critical for cellular functions like protein activity and cell volume.
Changes in ionic strength can result from signaling pathways or external osmolarity shifts.
Real-time monitoring tools for intracellular ionic strength are needed.

Purpose of the Study:

To develop a sensitive, real-time biosensor for intracellular ionic strength.
To utilize the Nano Luciferase (NanoLuc) protein for bioluminescence-based sensing.
To validate the sensor's performance in cell lysates and live cells.

Main Methods:

Biochemical characterization of NanoLuc protein's ionic strength-dependent bioluminescence.
Demonstration of reversible NanoLuc activity changes with ionic strength.
Development of an mNeonGreen-NanoLuc fusion protein for ratiometric measurements.

Main Results:

NanoLuc bioluminescence is sensitive to ionic strength across a broad range.
NanoLuc activity reduction at high ionic strength is reversible.
Ratiometric monitoring of ionic strength in cellular environments was achieved.

Conclusions:

A novel bioluminescence-based biosensing strategy for intracellular ionic strength was developed.
The mNeonGreen-NanoLuc fusion protein enables ratiometric sensing in live cells.
This tool has broad applications in cellular signaling, ion channel research, and drug discovery.