BIONOTE as an Innovative Biosensor for Measuring Endocannabinoid Levels

Area of Science:

• Analytical Chemistry
• Biotechnology
• Neuroscience

Background:

• Endocannabinoids (eCBs) play crucial roles in physiological processes.
• Accurate quantification of eCBs in biological matrices is challenging.
• Existing methods for eCB detection can be complex and costly.

Purpose of the Study:

• To develop and validate a novel liquid biosensor, BIONOTE, for quantifying endocannabinoids.
• To assess BIONOTE's ability to detect and differentiate key eCBs and their related molecules.
• To evaluate the potential of BIONOTE for analyzing eCBs in human plasma.

Main Methods:

• Utilized a BIONOTE liquid biosensor with an electrochemical probe functionalized with bovine serum albumin.
• Analyzed anandamide (AEA) and 2-arachidonoylglycerol (2-AG) in vitro.
• Tested BIONOTE's capacity to discriminate between AEA, 2-AG, and their moieties (arachidonic acid, ethanolamine, glycerol).
• Quantified AEA in human plasma samples.

Main Results:

• BIONOTE successfully detected AEA and 2-AG at low nanomolar concentrations.
• The biosensor effectively discriminated between AEA, 2-AG, and their respective moieties.
• BIONOTE demonstrated the ability to quantify AEA in human plasma.
• The functionalized probe enhanced the analytical system's sensitivity.

Conclusions:

• BIONOTE represents a novel, sensitive, and selective platform for endocannabinoid quantification.
• The biosensor is a user-friendly and cost-effective prototype for high-throughput analysis of eCBs.
• BIONOTE shows promise as a diagnostic tool for various medical applications.