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BIONOTE as an Innovative Biosensor for Measuring Endocannabinoid Levels.

Simone Grasso¹, Marco Santonico¹, Giorgio Pennazza²

¹Department of Science and Technology for Humans and the Environment, Campus Bio-Medico University of Rome, 00128 Rome, Italy.

Sensors (Basel, Switzerland)

|January 15, 2021

Summary

A new biosensor, BIONOTE, can quantify endocannabinoids (eCBs) like anandamide (AEA) and 2-arachidonoylglycerol (2-AG) in biological samples. This technology offers a user-friendly and cost-effective method for measuring eCB levels.

Keywords:

BIONOTE endocannabinoids liquid biosensor

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Area of Science:

Analytical Chemistry
Biotechnology
Neuroscience

Background:

Endocannabinoids (eCBs) play crucial roles in physiological processes.
Accurate quantification of eCBs in biological matrices is challenging.
Existing methods for eCB detection can be complex and costly.

Purpose of the Study:

To develop and validate a novel liquid biosensor, BIONOTE, for quantifying endocannabinoids.
To assess BIONOTE's ability to detect and differentiate key eCBs and their related molecules.
To evaluate the potential of BIONOTE for analyzing eCBs in human plasma.

Main Methods:

Utilized a BIONOTE liquid biosensor with an electrochemical probe functionalized with bovine serum albumin.
Analyzed anandamide (AEA) and 2-arachidonoylglycerol (2-AG) in vitro.
Tested BIONOTE's capacity to discriminate between AEA, 2-AG, and their moieties (arachidonic acid, ethanolamine, glycerol).
Quantified AEA in human plasma samples.

Main Results:

BIONOTE successfully detected AEA and 2-AG at low nanomolar concentrations.
The biosensor effectively discriminated between AEA, 2-AG, and their respective moieties.
BIONOTE demonstrated the ability to quantify AEA in human plasma.
The functionalized probe enhanced the analytical system's sensitivity.

Conclusions:

BIONOTE represents a novel, sensitive, and selective platform for endocannabinoid quantification.
The biosensor is a user-friendly and cost-effective prototype for high-throughput analysis of eCBs.
BIONOTE shows promise as a diagnostic tool for various medical applications.