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Updated: Nov 21, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating miR-499b-5p/RWDD4 Axis

Yanyan Zheng1, Jing Xie2, Xiaomin Xu1

  • 1Department of Neurology, Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou 325000, People's Republic of China.

Oncotargets and Therapy
|January 15, 2021
PubMed
Abstract

Insights

The long noncoding RNA DDX11-AS1 promotes glioma progression by interacting with miR-499b-5p, leading to increased RWDD4 expression. This finding offers potential therapeutic targets for glioma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) play critical roles in tumorigenesis.
  • The specific involvement of lncRNAs in glioma development is not well understood.

Purpose of the Study:

  • To investigate the role of DDX11-AS1 in glioma progression.
  • To elucidate the molecular mechanism by which DDX11-AS1 influences glioma.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • Patient survival analysis and in vitro functional assays (CCK8, colony formation, transwell).
  • Luciferase and RNA pulldown assays to determine molecular interactions.

Main Results:

  • DDX11-AS1 expression is significantly upregulated in glioma tissues and cells.
  • High DDX11-AS1 expression correlates with a poor prognosis in glioma patients.
  • Knockdown of DDX11-AS1 inhibits glioma cell proliferation, migration, and invasion, while promoting apoptosis.
  • DDX11-AS1 acts as a competing endogenous RNA (ceRNA) by sponging miR-499b-5p, leading to the upregulation of its target gene, RWDD4.
  • RWDD4 is upregulated, and miR-499b-5p is downregulated in glioma.

Conclusions:

  • Upregulation of DDX11-AS1 promotes glioma progression.
  • The DDX11-AS1/miR-499b-5p/RWDD4 axis is a key mechanism in glioma development.
  • DDX11-AS1 represents a potential therapeutic target for glioma.
Keywords:
DDX11-AS1RWDD4gliomamiR-499b-5p

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