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Low expression of long non-coding RNA ARAP1-AS1 can inhibit lung cancer proliferation by inducing G0/G1 cell cycle

Xinlu Tao1,2, Yan Zhang2, Jiaping Li2

  • 1Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China.

Journal of Thoracic Disease
|January 15, 2021
PubMed
Abstract

Insights

Long non-coding RNA ARAP1-AS1 is highly expressed in lung cancer. Inhibiting ARAP1-AS1 significantly reduces lung cancer cell proliferation and growth, offering potential therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Lung cancer is a leading cause of cancer-related mortality worldwide.
  • Long non-coding RNAs (lncRNAs) are emerging as critical regulators in various cancers, including lung cancer.
  • The specific role and molecular mechanisms of lncRNA ARAP1 antisense RNA 1 (ARAP1-AS1) in lung cancer remain largely unclear.

Purpose of the Study:

  • To investigate the expression and function of lncRNA ARAP1-AS1 in lung cancer.
  • To elucidate the molecular mechanism by which ARAP1-AS1 influences lung cancer development.
  • To evaluate ARAP1-AS1 as a potential molecular target for lung cancer diagnosis and therapy.

Main Methods:

  • Quantitative real-time PCR to assess ARAP1-AS1 expression in lung cancer tissues and cell lines.
  • Cell proliferation assays (MTT, plate cloning) and flow cytometry to evaluate the impact of ARAP1-AS1 knockdown on cell cycle progression.
  • Western blotting to analyze the expression of cell cycle-related proteins (cyclin D1, CDK4, CDK6).
  • Nude mouse xenograft models to assess the in vivo effect of ARAP1-AS1 knockdown on tumor growth.

Main Results:

  • ARAP1-AS1 was found to be significantly upregulated in lung cancer tissues and cell lines.
  • Knockdown of ARAP1-AS1 markedly inhibited lung cancer cell proliferation and colony formation in vitro.
  • ARAP1-AS1 knockdown induced G0/G1 phase cell cycle arrest and reduced cyclin D1 expression.
  • In vivo studies demonstrated that ARAP1-AS1 knockdown suppressed tumor growth and decreased Ki-67 expression in tumor tissues.

Conclusions:

  • LncRNA ARAP1-AS1 is oncogenic and overexpressed in lung cancer.
  • Downregulation of ARAP1-AS1 inhibits lung cancer cell proliferation and tumor growth both in vitro and in vivo.
  • ARAP1-AS1 may promote lung cancer progression by regulating the cell cycle, specifically through cyclin D1 inhibition.
Keywords:
Lung cancercell proliferationcycle arrestlong non-coding ribonucleic acid ARAP1 antisense RNA 1 (lncRNA ARAP1-AS1)nude mice

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