Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association between air pollution and incident cardiovascular diseases among a population with cardiovascular-kidney-metabolic syndrome stages 0-3: the first evidence from the China Health and Retirement Longitudinal Study.

Frontiers in endocrinology·2026
Same author

Latent profiles of nurses' attitudes toward artificial intelligence in nursing and associated factors: a cross-sectional study.

BMC nursing·2026
Same author

Inspired by the cocktail effect to prepare porous carbon with high removal performance: the critical role of sp<sup>3</sup>-C and CO in adsorption and degradation on porous carbon.

Bioresource technology·2026
Same author

Relaxed-tolerance polarization-insensitive all-optical wavelength conversion using SOA-FWM with weighting-assisted equalization.

Optics express·2026
Same author

Cellular prion protein suppresses colitis and colorectal carcinogenesis via modulating inflammatory responses.

Biochimica et biophysica acta. Molecular basis of disease·2026
Same author

CD163<sup>+</sup> macrophages coordinate erythroblastic Island formation and iron metabolism to enable glucocorticoid-induced erythropoiesis.

Biomarker research·2026

Related Experiment Video

Updated: Nov 21, 2025

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
09:25

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

15.6K

Cell cycle during neuronal migration and neocortical lamination.

Shanshan Wu1,2, Tingting Wei1, Wenjuan Fan3

  • 1National Health Commission, Key Laboratory of Birth Defect Prevention, Henan Scientific and Technical Institute of Reproductive Health, Zhengzhou, China.

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
|January 15, 2021
PubMed
Summary

Neural stem cells and migrating neurons exhibit active cell cycles during mouse cortical development, while mature pyramidal and Cajal-Retzius cells exit the cell cycle, impacting neocortical lamination.

Keywords:
cell cyclecortical laminationneocortexneuron migrationpyramidal cells

More Related Videos

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain
04:17

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain

Published on: March 8, 2024

1.3K
Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
13:33

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Published on: July 25, 2017

11.4K

Related Experiment Videos

Last Updated: Nov 21, 2025

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
09:25

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

15.6K
Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain
04:17

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain

Published on: March 8, 2024

1.3K
Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
13:33

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Published on: July 25, 2017

11.4K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Neocortical lamination is a complex process involving precise cell positioning.
  • Understanding the cell cycle status of different neuronal populations is crucial for deciphering developmental mechanisms.

Purpose of the Study:

  • To investigate the relationship between neocortical lamination and the cell cycle.
  • To determine the cell cycle phases of various cell types during mouse cortical development.

Main Methods:

  • Immunofluorescent labeling, BrdU assay, and DiI tracing were used in mouse neocortex and hippocampus.
  • Specific protein markers (FOXP2, CDP, Nestin) were identified for cortical lamination.

Main Results:

  • Neural stem cells in the subventricular zone express markers indicating active cell cycling (Nestin, Cyclin A2, Cyclin E1, CDT1).
  • Migrating postmitotic neurons in the neocortex are in the G1 phase (Cyclin D1 positive).
  • Mature pyramidal cells and Cajal-Retzius (CR) cells in the cortical plate and molecular layer, respectively, have exited the cell cycle (Cyclin D1 negative, G0 phase).

Conclusions:

  • Cell cycle regulation is integral to neural migration, proliferation, and neocortical lamination.
  • Distinct cell populations within the developing neocortex display differential cell cycle engagement.
  • Pyramidal cells and CR cells cease proliferation to achieve their mature states during cortical development.