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Surface-Functionalized Conducting Nanofibers for Electrically Stimulated Neural Cell Function.

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Surface-functionalized electroconductive nanofibers enhance nerve regeneration. Combining chemical cues with electrical stimulation accelerates neuronal growth, offering a promising strategy for neural scaffolds.

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Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Polymer Chemistry

Background:

  • Enhancing nerve regeneration requires biomaterials with cell-instructive cues and physical stimuli.
  • Poly(2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene) (MEH-PPV) based nanofibers offer electroconductive properties for neural applications.
  • Surface functionalization is crucial for improving cell adhesion and differentiation on biomaterial scaffolds.

Purpose of the Study:

  • To investigate the surface functionalization of MEH-PPV electroconductive nanofibers for accelerated neuronal growth.
  • To explore the voltage-dependent conductive mechanism of nanofibers and its link to electrically stimulated neuronal responses.
  • To evaluate the efficacy of surface amination as a cost-effective alternative to biomolecule coating for neural regeneration.

Main Methods:

  • Fabrication of MEH-PPV-based electrospun nanofibers.
  • Surface functionalization using 3-aminopropyltriethoxysilane (APTES) and 1,6-hexanediamine (HDA).
  • Characterization of nanofiber properties (uniformity, porosity, conductivity, mechanical strength).
  • In vitro evaluation of cell adhesion, spreading (3T3 fibroblasts), and differentiation (PC12 neuronal cells).
  • Assessment of neurite formation and elongation under electrical stimulation (ES).

Main Results:

  • Uniform, porous, conductive, and mechanically robust nanofibers were successfully fabricated.
  • Surface amination significantly enhanced fibroblast adhesion and spreading.
  • Neuronal PC12 cells exhibited improved differentiation on aminated nanofibers.
  • Combined surface amination and ES promoted neurite formation and elongation comparable or superior to collagen-coated scaffolds without ES.

Conclusions:

  • Surface amination of MEH-PPV nanofibers improves biocompatibility and neuronal differentiation.
  • MEH-PPV nanofibers coupled with electrical stimulation represent a promising strategy for nerve regeneration.
  • This approach offers a potentially cost-effective alternative to traditional biomolecule coatings for neural scaffolds.