Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Korean red ginseng on deep capillary plexus parameters in diabetic retinopathy: A prospective, randomized, double-blind clinical trial.

Journal of ginseng research·2026
Same author

Ta Phase Engineering for Defect-Controlled Reliable Switching in Ultrathin TaO<i><sub>x</sub></i> Memristors.

ACS applied materials & interfaces·2026
Same author

Biallelic PKD1 mutations causing neonatal death in an extremely preterm infant: a Korean case report of very early-onset ADPKD.

BMC nephrology·2026
Same author

Imparting Biodegradability to Highly-Efficient Upconversion Nanoparticles via Facet-Selective Zirconium Doping.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Reinforcement of Cathode Interface Using a Dipolar Small Molecule for Enhancing Operational Stability of Perovskite Solar Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Long-Term Mortality Following Hepatitis C Cure in a Real-World Multinational Cohort.

Liver international : official journal of the International Association for the Study of the Liver·2026

Related Experiment Video

Updated: Nov 21, 2025

Synthesis of Gold Nanoparticle Integrated Photo-responsive Liposomes and Measurement of Their Microbubble Cavitation upon Pulse Laser Excitation
12:00

Synthesis of Gold Nanoparticle Integrated Photo-responsive Liposomes and Measurement of Their Microbubble Cavitation upon Pulse Laser Excitation

Published on: February 24, 2016

8.3K

Solid-state colorimetric polydiacetylene liposome biosensor sensitized by gold nanoparticles.

Jayoung Kim1, Byeong-Seok Moon, Eunhee Hwang

  • 1School of Chemical Engineering, Sungkyunkwan University, 16419, Republic of Korea. dhkim1@skku.edu.

The Analyst
|January 15, 2021
PubMed
Summary

This study introduces a novel solid-state biosensor using polydiacetylene (PDA) liposomes and gold nanoparticles (AuNPs) for enhanced colorimetric detection of biomolecules, improving sensitivity for point-of-care diagnostics.

More Related Videos

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

13.7K
Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
12:23

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

14.7K

Related Experiment Videos

Last Updated: Nov 21, 2025

Synthesis of Gold Nanoparticle Integrated Photo-responsive Liposomes and Measurement of Their Microbubble Cavitation upon Pulse Laser Excitation
12:00

Synthesis of Gold Nanoparticle Integrated Photo-responsive Liposomes and Measurement of Their Microbubble Cavitation upon Pulse Laser Excitation

Published on: February 24, 2016

8.3K
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

13.7K
Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
12:23

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

14.7K

Area of Science:

  • Polymer Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Polydiacetylene (PDA) exhibits colorimetric changes upon biomolecule binding, ideal for biosensors.
  • Solid-state PDA biosensors for point-of-care use are underdeveloped.
  • Current PDA biosensors lack sufficient sensitivity for some diagnostic applications.

Purpose of the Study:

  • To develop a highly sensitive solid-state biosensor based on PDA liposomes.
  • To enhance the colorimetric response of PDA biosensors using gold nanoparticles (AuNPs).
  • To demonstrate the utility of this biosensor for detecting specific biomolecules like thrombin.

Main Methods:

  • Functionalization of PDA liposomes with gold nanoparticles (AuNPs) and a thrombin-binding aptamer.
  • Attachment of functionalized PDA liposomes to a substrate for solid-state detection.
  • Colorimetric analysis of thrombin binding to the functionalized biosensor.

Main Results:

  • The developed solid-state biosensor showed enhanced sensitivity for thrombin detection.
  • Sensitivity was increased 2.5-fold with the inclusion of AuNPs compared to PDA liposomes alone.
  • AuNPs act as signal sensitizers by inducing steric repulsion and further distorting the PDA backbone.

Conclusions:

  • The novel solid-state PDA liposome biosensor with AuNPs significantly enhances detection sensitivity.
  • The target-independent steric repulsion mechanism of AuNPs offers a versatile approach for biosensor development.
  • This technology paves the way for advanced, highly sensitive solid-state biosensors for point-of-care applications.