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Liver Histology

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The microscopic anatomy of the liver is a complex and intricate system that comprises numerous structural units known as liver lobules, each of which is comparable in size to a sesame seed. These hexagonal structures consist of plates of liver cells or hepatocytes, which are characterized by their versatility and abundance of cellular apparatus like rough and smooth ER, Golgi apparatus, peroxisomes, and mitochondria.
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Updated: Nov 21, 2025

Decellularization and Recellularization of Whole Livers

Decellularization and Recellularization of Whole Livers

Published on: February 4, 2011

Functionalized Vascular Structure in Bioengineered Liver Identified with Proteomics.

Beibei Guo¹, Qian Zhou¹, Jing Jiang^1,2

¹State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.

ACS Biomaterials Science & Engineering

|January 15, 2021

Summary

This summary is machine-generated.

Developing a functional bioengineered liver requires vascularization. Proteomics revealed distinct biological processes, with 14 days of culture optimal for creating functional vascular structures in vitro.

Keywords:

Bioengineered liver decellularized liver scaffold proteomics vascularization

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Area of Science:

Tissue Engineering
Regenerative Medicine
Proteomics

Background:

Vascularization is a critical hurdle in creating functional bioengineered livers.
Understanding the dynamic biological processes of vascularization is essential for improving liver tissue engineering.

Purpose of the Study:

To develop a functionalized vascular structure within a bioengineered liver.
To identify the temporal biological vascularization processes using proteomics.

Main Methods:

Decellularized rat liver scaffolds were seeded with human umbilical vein endothelial cells (HUVECs).
Vascularization was assessed over 21 days with proteomics analysis at multiple time points.
Key protein expression and biological pathways were analyzed.

Main Results:

HUVECs formed a functional barrier within 7 days, with sustained biological activity for over 21 days.
Proteomics revealed distinct protein expression profiles after 14 days, shifting from cell adhesion and metabolism to muscle contraction and filament sliding.
Identified biological processes included angiogenesis, myogenesis, and vascular function, supported by basement membrane assembly and junction regulation.

Conclusions:

Proteomics analysis provides insights into the temporal development of vascular structures in bioengineered livers.
A minimum of 14 days of in vitro culture is recommended for developing functionalized vascular structures.
This research aids in understanding vascularization mechanisms for future clinical applications of bioengineered livers.