Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combining electroacupuncture and transcutaneous electrical acupoint stimulation for psychiatric disorders in women victims of domestic violence: An assessor-blinded, randomised controlled trial.

General psychiatry·2026
Same author

Rapid Progression of Coronary Disease in Fabry Disease: Vulnerable Plaque by Intravascular Ultrasound.

JACC. Case reports·2026
Same author

Evaluation of <i>Pseudomonas chlororaphis</i> JK77 as a biocontrol agent for <i>Fusarium graminearum</i> in maize stalk rot management.

Plant disease·2026
Same author

Impact of origin and frequency of premature ventricular complexes on cardiac remodeling: a systematic review and meta-analysis.

Journal of cardiothoracic surgery·2026
Same author

Neutralizing LFA-1 alleviates acute lung injury by diminishing pulmonary retention of CAR-T cells.

Pharmacological research·2026
Same author

Neural Network Enabled Real-Time Plasma Imaging for Inverse Designed Fabrication of Micro/Nano Structures with Ultrafast Laser.

Advanced materials (Deerfield Beach, Fla.)·2026

Related Experiment Video

Updated: Nov 21, 2025

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Published on: February 28, 2019

9.3K

Microprocessing on Single Protein Crystals Using Femtosecond Pulse Laser.

Jiachen Yu1, Lan Jiang2, Jianfeng Yan1

  • 1Key Laboratory for Advanced Materials Processing Technology, Ministry of Education of China, State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.

ACS Biomaterials Science & Engineering
|January 15, 2021
PubMed
Summary
This summary is machine-generated.

Femtosecond laser processing enables precise micropatterning on fragile protein crystals without damage. This technique creates intricate patterns for biosensing and biodevices, overcoming previous limitations.

Keywords:
femtosecond lasermicroprocessingprotein crystal

More Related Videos

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
09:09

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Published on: September 20, 2016

11.7K
Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
08:44

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

7.9K

Related Experiment Videos

Last Updated: Nov 21, 2025

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Published on: February 28, 2019

9.3K
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
09:09

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Published on: September 20, 2016

11.7K
Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
08:44

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

7.9K

Area of Science:

  • Materials Science
  • Biotechnology
  • Laser Physics

Background:

  • Micropatterning proteins is crucial for biosensing and biomedical applications.
  • Protein crystal fragility poses challenges for conventional microfabrication techniques.

Purpose of the Study:

  • To investigate femtosecond laser processing for creating micropatterns on single hen egg white lysozyme protein crystals.
  • To optimize laser parameters for precise micropatterning without inducing crystal damage.

Main Methods:

  • Utilized femtosecond laser ablation on protein crystals.
  • Investigated the influence of laser fluence and pulse number on ablation morphology.
  • Performed numerical simulations to understand ionization and energy deposition processes.

Main Results:

  • Achieved micropatterning on protein crystals with a minimum feature size of 2 μm.
  • Identified optimized laser parameters to prevent crystal cracking.
  • Observed distinct ablation morphologies (holes or foaming) based on laser fluence and pulse count.

Conclusions:

  • Femtosecond laser processing is a viable method for creating complex micropatterns on protein crystals.
  • The developed technique supports applications in biosensing microarrays and enhanced crystal growth.
  • This microprocessing approach holds promise for advancements in biodevices and biomedicine.