Related Experiment Video
Updated: Nov 21, 2025

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
Published on: December 23, 2022
Effects of Debranching on the Formation of Maize Starch-Lauric Acid-β-Lactoglobulin Complexes
Jingjing Cai1,2, Chen Chao1,2, Bin Niu1
1State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, People's Republic of China.
Abstract:
In this study, the effects of debranching on the structure and properties of the starch-lauric acid (LA)-β-lactoglobulin (βLG) complex were studied. Gel permeation chromatography and high-performance anion-exchange chromatography showed that debranching of amylopectin generated short linear chains, which increased in proportions with debranching time. Analyses from differential scanning calorimetry, laser confocal micro-Raman spectroscopy, and X-ray diffraction showed that debranching promoted the formation of starch-LA and starch-LA-βLG complexes, as characterized by the increased enthalpy changes and crystallinity and decreased full width at half maximum of the band at 480 cm-1. Debranching treatment for 6 and 18 h promoted complexation between starch and LA, while extensive debranching was unfavorable for the formation of starch-LA complexes. Similar results were also observed for the starch-LA-βLG complexes. Starch-LA-βLG complexes had more type II and less type I crystallites than starch-LA complexes. From this study, we conclude that debranching of starch favors the formation of starch-LA and starch-LA-βLG complexes, with more type II crystallites formed in starch-LA-βLG complexes.
More Related Videos
Related Concept Videos
Formation of Lipopolysaccharides
Hydrolysis
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
Lipid Digestion
Carbohydrate Digestion
Mass Spectrometry: Branched Alkane Fragmentation
Loss of Carboxy Group as CO2: Decarboxylation of Malonic Acid Derivatives

