A Sensitive Single Particle-ICP-MS Method for CeO₂ Nanoparticles Analysis in Soil during Aging Process

The increasing prevalence of products that incorporate engineered nanoparticles (ENPs) has prompted efforts to investigate the potential release, environmental fate, and exposure of the ENPs. However, the investigation of cerium dioxide nanoparticles (CeO₂ NPs) in soil has remained limited, owing to the analytical challenge from the soil's complex nature. In this study, this challenge was overcome by applying a novel single particle-inductively coupled plasma-mass spectrometry (SP-ICP-MS) methodology to detect CeO₂ NPs extracted from soil, utilizing tetrasodium pyrophosphate (TSPP) aqueous solution as an extractant. This method is highly sensitive for determining CeO₂ NPs in soil, with detection limits of size and concentration of 15 nm and 194 NPs mL^-1, respectively. Extraction efficiency was sufficient in the tested TSPP concentration range from 1 mM to 10 mM at a soil-to-extractant ratio 1:100 (g mL^-1) for the extraction of CeO₂ NPs from the soil spiked with CeO₂ NPs. The aging study demonstrated that particle size, size distribution, and particle concentration underwent no significant change in the aged soils for a short period of one month. This study showed an efficient method capable of extracting and accurately determining CeO₂ NPs in soil matrices. The method can serve as a useful tool for nanoparticle analysis in routine soil tests and soil research.