Different chemometric assisted approaches for spectrophotometric quantitative analysis of lesinurad and allopurinol

Three chemometric assisted spectrophotometric approaches were designed for precise quantitative analysis of lesinurad and allopurinol, in their recently FDA approved combination pharmaceutical dosage form. By utilizing the recorded absorption spectral data of lesinurad and allopurinol mixtures, principle component regression, partial least squares and genetic algorithm partial least squares were developed and compared for the spectrophotometric quantitative analysis of the cited drugs in their binary mixture. Experimental design for different concentrations of the studied drugs was done based on the spectral sensitivity and the commercial ratio of the two drugs in the combined pharmaceutical dosage form. Optimization of the described models was done using five-levels, two factors experimental design principle. Quantitative assay of the two drugs in Duzallo® tablet was successfully done and the data obtained was statistically compared with the results of another published HPLC quantitative analytical method.