Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.1K
Transgenic Plants02:50

Transgenic Plants

8.1K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.1K
CRISPR01:59

CRISPR

55.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
55.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intravenous MR Lymphangiography: Feasibility in a Swine Model and Proof-of-Concept in Patients.

Radiology·2026
Same author

Effects of metal components in PM<sub>2.5</sub> derived from pig farm exposure on ovarian function and oocyte quality.

Journal of hazardous materials·2026
Same author

Resting-state EEG default mode network connectivity and suicidal ideation in depressive disorders: a retrospective cross-sectional study.

BMC psychiatry·2026
Same author

Rapid and reliable computational markers of decision-making for predicting daily smoking behavior and smoking cessation treatment outcomes.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026
Same author

Cytogenetic insights into the diversification of Calanthe: polyploidy, karyotype variation, and genome size evolution.

BMC plant biology·2026
Same author

Vemurafenib Induces Apoptosis via JNK Activation and AKT Inhibition in Hepatocellular Carcinoma.

Journal of Cancer·2026

Related Experiment Video

Updated: Nov 21, 2025

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
05:56

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Published on: February 27, 2021

5.5K

Virus-induced plant genome editing.

Youngbin Oh1, Hyeonjin Kim1, Sang-Gyu Kim1

  • 1Department of Biological Sciences, Korea Advanced Institute for Science and Technology, Daejeon 34141, Republic of Korea.

Current Opinion in Plant Biology
|January 15, 2021
PubMed
Summary

Virus vectors enable CRISPR gene editing in plants by delivering Cas proteins and guide RNAs. This technology, virus-induced genome editing (VIGE), offers a simpler method for creating mutant seeds.

More Related Videos

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato
06:34

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato

Published on: June 10, 2009

53.4K
Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.9K

Related Experiment Videos

Last Updated: Nov 21, 2025

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
05:56

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Published on: February 27, 2021

5.5K
Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato
06:34

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato

Published on: June 10, 2009

53.4K
Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.9K

Area of Science:

  • Plant biotechnology
  • Molecular biology
  • Genetics

Background:

  • Plant viruses have been engineered for decades to express foreign proteins and RNAs.
  • This viral system is now being adapted for plant genome editing applications.

Purpose of the Study:

  • To explore the application of virus vectors for plant genome editing.
  • To address key challenges in virus-induced genome editing (VIGE) technology in plants.

Main Methods:

  • Utilizing plant virus vectors to deliver CRISPR-Cas components (Cas proteins and guide RNAs) into plant cells.
  • Investigating methods to express large Cas proteins and guide RNAs within the viral system.
  • Exploring strategies to enhance virus delivery to meristematic cells for efficient editing.

Main Results:

  • Virus vectors can deliver CRISPR gene-editing components into plant cells, bypassing complex experimental procedures.
  • In some cases, viruses move to meristematic cells, leading to gene editing and the production of mutant seeds.
  • Recent advances focus on overcoming challenges in Cas protein expression, guide RNA expression, and viral delivery efficiency.

Conclusions:

  • Virus-induced genome editing (VIGE) presents a promising and simplified approach for editing plant genomes.
  • Further research is enhancing the efficiency and applicability of plant virus vectors for precise genome modification in plants.