Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Decade of Leadership and Impact: Celebrating 10 Years of the ORS Spine Section.

JOR spine·2026
Same author

Comparing Movement Patterns and Physical Function Between Chronic Low Back Pain Patients With Nociplastic and Nociceptive Pain Categories.

JOR spine·2026
Same author

Sex-Based Differences in Cell Types and Gene Expression within the Anterior Cruciate Ligament.

The Journal of bone and joint surgery. American volume·2026
Same author

Vertebral Growth Modulation Through Periosteal Resection and Fixed Length Deformity Overcorrection: Computational and In Vivo Pilot Study.

JOR spine·2025
Same author

Corneal Sensory Receptors and Pharmacological Therapies to Modulate Ocular Pain.

International journal of molecular sciences·2025
Same author

Characterization of the murine spine for spaceflight studies.

PloS one·2025

Related Experiment Video

Updated: Nov 21, 2025

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
08:34

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

17.1K

Agarose-Based Hydrogels as Suitable Bioprinting Materials for Tissue Engineering.

Gabriel R López-Marcial1, Anne Y Zeng1, Carlos Osuna2

  • 1Department of Mechanical Engineering, University of California, Berkeley, California 94720, United States.

ACS Biomaterials Science & Engineering
|January 16, 2021
PubMed
Summary

New agarose-alginate bioinks show promise for 3D printing cell-laden tissue scaffolds. These materials offer excellent print fidelity and cell viability, supporting cartilage tissue engineering applications.

Keywords:
3D printingadditive manufacturingagarosealginatebioinksbioprintinghydrogels

More Related Videos

Author Spotlight: Development of Homogeneous κ-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting
04:58

Author Spotlight: Development of Homogeneous κ-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting

Published on: May 3, 2024

1.5K
Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation
16:20

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation

Published on: July 2, 2018

19.1K

Related Experiment Videos

Last Updated: Nov 21, 2025

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
08:34

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

17.1K
Author Spotlight: Development of Homogeneous κ-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting
04:58

Author Spotlight: Development of Homogeneous κ-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting

Published on: May 3, 2024

1.5K
Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation
16:20

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation

Published on: July 2, 2018

19.1K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Additive Manufacturing

Background:

  • Hydrogels are essential scaffolds in tissue engineering but often require additional techniques for 3D printing, which can impede cell growth.
  • A need exists for advanced bioinks enabling direct printing of cell-laden constructs with high fidelity.

Purpose of the Study:

  • To evaluate agarose-alginate hydrogel mixtures as potential bioinks for cell-based tissue engineering.
  • To compare the printability and mechanical properties of these novel mixtures against established hydrogels like Pluronic and agarose.

Main Methods:

  • Comparative analysis of rheological properties (yield stress, storage modulus, shear thinning) and mechanical characteristics.
  • Assessment of construct shape fidelity and cell viability over a 28-day culture period for printed constructs.
  • Development and testing of alginate-agarose composite hydrogels for bioprinting applications.

Main Results:

  • Agarose-alginate gels exhibited rheological properties and printability statistically similar to Pluronic (p > 0.05).
  • A 5% w/v alginate-agarose composite (3:2 ratio) demonstrated over 70% cell survival at 28 days.
  • Significant matrix production was observed in the alginate-agarose composite constructs over the culture period.

Conclusions:

  • Agarose-alginate hydrogel mixtures represent a promising bioink for additive manufacturing in tissue engineering.
  • These composite materials support cell viability and matrix production, making them suitable for cartilage tissue engineering scaffolds.