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Updated: Nov 21, 2025

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Near-Ultraviolet Circular Dichroism and Two-Dimensional Spectroscopy of Polypeptides
Francesco Segatta1, David M Rogers2, Naomi T Dyer2
1Dipartimento di Chimica Industriale "Toso Montanari", Universita' degli Studi di Bologna, Viale del Risorgimento, 4, I-40136 Bologna, Italy.
Abstract:
A fully quantitative theory of the relationship between protein conformation and optical spectroscopy would facilitate deeper insights into biophysical and simulation studies of protein dynamics and folding. In contrast to intense bands in the far-ultraviolet, near-UV bands are much weaker and have been challenging to compute theoretically. We report some advances in the accuracy of calculations in the near-UV, which were realised through the consideration of the vibrational structure of the electronic transitions of aromatic side chains.
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