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Microfluidic sperm selection yields higher sperm quality compared to conventional method in ICSI program: A pilot

Fatemeh Anbari1,2, Mohammad Ali Khalili1,2, Abdul Munaf Sultan Ahamed3

  • 1Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Systems Biology in Reproductive Medicine
|January 16, 2021
PubMed
Summary
This summary is machine-generated.

Microfluidic Sperm Sorting (MSS) significantly improves sperm quality and DNA integrity for intracytoplasmic sperm injection (ICSI), leading to better embryo development and higher pregnancy rates compared to the Direct Swim Up (DSU) method.

Keywords:
ICSIMSOMEMicrofluidic sperm sorting (MSS)direct swim upembryo development

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Area of Science:

  • Reproductive Medicine
  • Sperm Biology
  • Assisted Reproductive Technology

Background:

  • High-quality sperm preparation is crucial for successful intracytoplasmic sperm injection (ICSI).
  • Existing methods like Direct Swim Up (DSU) may not consistently yield optimal sperm for ICSI.
  • Microfluidic Sperm Sorting (MSS) offers a novel approach to sperm selection.

Purpose of the Study:

  • To evaluate the efficacy of Microfluidic Sperm Sorting (MSS) for preparing spermatozoa for ICSI.
  • To compare sperm quality, DNA integrity, embryo development, and clinical outcomes between MSS and DSU methods.

Main Methods:

  • A comparative study involving 95 ICSI cases using sperm prepared by MSS or DSU.
  • Sperm quality parameters, including motility and morphology (MSOME), were assessed.
  • Sperm DNA fragmentation (SDF) was evaluated using the Sperm Chromatin Dispersion (SCD) test.

Main Results:

  • MSS significantly enhanced progressive motility and Class I sperm morphology compared to DSU (P < 0.01 and P < 0.001).
  • MSS demonstrated significantly lower rates of DNA fragmentation and immotile spermatozoa (P < 0.001).
  • Higher rates of high-quality embryo formation (P < 0.001), implantation (P = 0.04), and pregnancy (P = 0.05) were observed with MSS.

Conclusions:

  • Microfluidic Sperm Sorting (MSS) is an effective, non-invasive, and accessible technique for isolating high-quality spermatozoa for ICSI.
  • MSS improves both laboratory parameters and clinical outcomes in assisted reproductive technology.
  • The MSS technique offers advantages in terms of ease of use and cost-effectiveness for sperm preparation.