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Benchtop micro-mashing: high-throughput, robust, experimental beer brewing.

Edward D Kerr1, Christopher H Caboche1, Peter Josh1

  • 1School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, 4072, Australia.

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|January 16, 2021
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Summary
This summary is machine-generated.

A new micro-scale mash method enables high-throughput brewing experiments using common lab equipment. This technique yields results comparable to traditional methods, facilitating detailed analysis of brewing proteomes and parameters.

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Area of Science:

  • Brewing Science
  • Analytical Chemistry
  • Proteomics

Background:

  • Modern analytical techniques are revolutionizing brewing science.
  • Traditional brewing experiments are often time-consuming, ingredient-intensive, and require specialized equipment.
  • The need for adequate replication in experimental design presents challenges for high-throughput analysis.

Purpose of the Study:

  • To develop a micro-scale mash method for high-throughput brewing analysis.
  • To enable easy sample replication for robust statistical analysis.
  • To investigate the effects of altered mash parameters on the beer brewing proteome.

Main Methods:

  • A micro-scale mash method was developed using a laboratory benchtop shaker/incubator.
  • Proteomic profiles were analyzed at both protein and peptide levels.
  • Comparisons were made between 1 mL micro-mashes and 23 L Braumeister mashes.
  • Fermentable sugar and free amino acid profiles of wort were analyzed.

Main Results:

  • Proteomic profiles were consistent between micro-scale and traditional mash scales.
  • Wort from both mash scales exhibited equivalent fermentable sugar and free amino acid profiles.
  • The micro-mash method allowed for high-throughput analysis and easy sample replication.
  • The flexibility of the micro-mash enabled investigation of altered mash parameters.

Conclusions:

  • The micro-scale mash method is a viable and efficient alternative for brewing research.
  • This method supports high-throughput experimentation and statistical rigor in brewing science.
  • It facilitates deeper understanding of the beer brewing proteome and mash parameter effects.