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High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
Published on: March 5, 2022
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Insight into different host range of three planthoppers by transcriptomic and microbiomic analysis.
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.
Insect Molecular Biology
|January 16, 2021
Summary
Small brown planthopper (SBPH) and white-backed planthopper (WBPH) adapt better to wheat than brown planthopper (BPH). Host plant affects planthopper gene expression and bacterial communities, revealing species-specific adaptations.
Area of Science:
- * Entomology and Molecular Biology: Investigating insect-plant interactions at the transcriptomic and microbial levels.
Background:
- * Brown planthopper (BPH), white-backed planthopper (WBPH), and small brown planthopper (SBPH) are closely related rice pests exhibiting differential performance on wheat.
- * Previous studies compared transcriptomic responses of SBPH and BPH on wheat, but WBPH responses and host plant influences on bacterial communities remained less understood.
Purpose of the Study:
- * To elucidate the molecular mechanisms underlying differential fitness of WBPH, BPH, and SBPH on rice versus wheat.
- * To investigate the impact of host plants on the bacterial communities associated with these three planthopper species.
Main Methods:
- * Transcriptomic analysis of WBPH on rice and wheat.
- * Comparative transcriptomic analysis of WBPH, BPH, and SBPH.
- * Analysis of bacterial communities in the three planthopper species across different host plants.
Main Results:
- * WBPH showed enriched genes related to detoxification, transportation, and proteasome pathways when feeding on different hosts.
- * Comparative transcriptomics revealed distinct co-regulated gene expression patterns between BPH/SBPH and similar patterns between BPH/WBPH.
- * While planthoppers harbored diverse bacterial communities, host plants did not significantly alter bacterial diversity or relative abundance.
Conclusions:
- * Host plant adaptation in planthoppers involves both generic and species-specific transcriptomic changes.
- * Bacterial communities within planthoppers appear resilient to host plant variations.
- * This research enhances understanding of planthopper host fitness mechanisms and adaptive strategies.
