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Published on: August 3, 2018
Identification of hsa-miR-106a-5p as an impact agent on promotion of multiple sclerosis using multi-step data
Samira Rahimirad1,2, Mohammad Navaderi1,2, Shokoofeh Alaei3
1Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Abstract:
Multiple sclerosis (MS) is a chronic, demyelinating disease in which the neuron myelin sheath is disrupted and leading to signal transductions disabilities. The evidence demonstrated that gene expression patterns and their related regulating factors are the most critical agents in MS demyelinating process. A miRNA is a small non-coding RNA which functions in post-transcriptional regulation of gene expression. Identification of specific miRNA dysregulation patterns in MS blood samples compared to healthy control can be used as a diagnostic and prognostic agent. Through the literature review and bioinformatics analysis, it was found that the hsa-miR-106a-5p can be considered a significant MS pathogenic factor, which seems has an abnormal expression pattern in patients' blood. Experimental validation using real-time PCR assay was carried to verifying the miR-106a-5p expression in MS and healthy control blood samples. The obtained results proved the miR-106a dysregulation in MS patients. The expression levels of miR-106a-5p were significantly downregulated (log 2 fold change = - 1.15) in patient blood samples compared to controls (p = 0.055). Our study suggested that miR-106a-5p may have a biomarker potential to the diagnosis of MS patients based on its dysregulation patterns.
Insights
MicroRNA-106a-5p is downregulated in multiple sclerosis (MS) patients. This finding suggests miR-106a-5p may serve as a potential biomarker for diagnosing MS through blood analysis.
Area of Science:
- Neuroscience
- Genetics
- Biochemistry
Background:
- Multiple sclerosis (MS) is a chronic demyelinating disease affecting the central nervous system.
- Gene expression and regulatory factors are critical in MS pathogenesis.
- MicroRNAs (miRNAs) are key post-transcriptional regulators of gene expression.
Purpose of the Study:
- To identify specific miRNA dysregulation patterns in MS patients for diagnostic potential.
- To investigate the role of hsa-miR-106a-5p as a pathogenic factor in MS.
Main Methods:
- Literature review and bioinformatics analysis to identify candidate miRNAs.
- Experimental validation using real-time PCR assay on blood samples from MS patients and healthy controls.
Main Results:
- Bioinformatics analysis identified hsa-miR-106a-5p as a significant factor in MS.
- Real-time PCR confirmed significant downregulation of miR-106a-5p in MS patients' blood (log2 fold change = -1.15, p=0.055).
Conclusions:
- Dysregulation of miR-106a-5p is evident in MS patients.
- miR-106a-5p shows potential as a diagnostic biomarker for multiple sclerosis.
