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Updated: Nov 21, 2025

Mesocosm-Scale Constructed Wetland Design for Wastewater Treatment
Published on: May 2, 2025
Hydraulic flow direction alters nutrients removal performance and microbial mechanisms in electrolysis-assisted
Yingmu Wang1, Jian Zhou2, Shuohui Shi2
1College of Civil Engineering, Fuzhou University, Fujian 350116, China; Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
Abstract:
In this study, an electrolysis-assisted down-flow constructed wetland (E-DFCW) was successfully established, and achieved simultaneously efficient removal of PO43--P (93.6% ± 3.2%), NO3--N (97.1% ± 2.0%) and TN (80.6% ± 5.4%). When compared with electrolysis-assisted up-flow constructed wetland (E-UFCW), E-DFCW allowed significantly lower concentrations of PO43--P, NO3--N, total Fe and SO42--S in effluents. In addition, microbial community and functional genes prediction results indicated that hydraulic flow direction significantly altered microbial nitrogen, sulfur and carbon metabolisms in electrolysis-assisted constructed wetlands (E-CWs). Specifically, multi-path denitrification facilitated NO3--N reduction in cathodic chamber of E-DFCW, whereas autohydrogenotrophic denitrification might dominate NO3--N reduction in cathodic chamber of E-UFCW. More abundant and diverse denitrifiers in cathodic chamber of E-DFCW contributed to enhanced denitrification performance. Overall, this work provides microbial insights into multi-path nitrogen metabolisms in electrolysis-assisted denitrification systems in response to hydraulic flow direction.
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