Intrinsic dual-emissive carbon dots for efficient ratiometric detection of Cu²⁺ and aspartic acid

Herein, novel intrinsic dual-emitting carbon dots (CDs) are prepared through a one-step hydrothermal treatment of glucose and 3-nitroaniline in sulfuric acid solution and utilized for ratiometric determination of Cu²⁺ and aspartic acid (Asp). The CDs exhibited an interesting pH-switchable emission behavior displaying an intrinsic dual-emitting peak with emission maxima at 400 and 610 nm at pH 4.0-5.0. The presence of Cu²⁺ intensively quenched the first emission peak at 400 nm, but it had a negligible effect on the second emission peak. The ratiometric signal displayed a high selectively for Cu²⁺ over other metal ions and provided a linear response over the concentration range of 0.01-1.00 μM with a detection limit of 7.0 nM. Moreover, at pH 4.0, Asp was able to restore the quenched fluorescence of the CDs-Cu²⁺ system with a much more successful performance than other amino acids. This on-off-on fluorescence behavior provided a selective ratiometric fluorescence method for the determination of Asp in the concentration range of 0.2-15 μM. The acceptable detection results for Cu²⁺ in a river water sample (compared to Inductively Coupled Plasma (ICP) method) and for Asp in human serum samples confirmed the potential application of this ratiometric nanoprobe for sensing in real samples.