Short communication: Context matters: Adult size is contingent on embryonic temperature in Drosophila melanogaster

Temperature is a critical factor in shaping ectothermic development. Developmental temperature may constrain, alter, or redirect phenotypes expressed later in life. Recent studies have begun to analyze the consequences of mismatches between developmental and adult environments. Few studies analyze the consequences environmental mismatches during development yield on adult phenotypes. The aim of this study was to determine how mismatched temperatures during development affect adult size in Drosophila melanogaster. We employed a full factorial design in which eggs were incubated for 24 h in one of two temperature treatments (18 °C or 28 °C) with half of the flies subsequently being switched to the opposite temperature treatment for the remainder of development. We measured body size shortly after eclosure. We found that variation in size after eclosure was contingent upon the temperature during the embryo stage. Flies reared initially in 18 °C eclosed larger regardless of the subsequent temperature until eclsoure. Flies reared initially in 28 °C, however, eclosed smaller only if they remained in 28 °C until eclosure. The degree of plasticity in size was therefore contingent upon temperature during the embryo stage. We discuss the implications of employing full factorial approaches to consider the full context of phenotypic outcomes in light of changing developmental environments.