Construction and Biocompatibility of Three-Dimensional Composite Polyurethane Scaffolds in Liquid Crystal State

Liquid crystal (LC), a characteristic substance of biofilms, has been reported to positively affect cell affinity. To better combine and utilize the properties of an LC and the advantages of polyurethane (PU) elastomers, the three-dimensional printing (3DP) molding technology and the simple soaking-swelling blending technology were used to construct PU/LC 3D composite scaffolds, and the compressive strength, porosity, hydrophilicity, and in vitro cell experiments of the scaffolds were initially discussed. The results indicated that the newly developed PU/LC 3D composite scaffolds exhibited an LC state; the addition of an LC did not change the porosity after swelling while maintaining a high porosity; the compressive strength of the composite scaffolds decreased while still maintaining high mechanical properties and enhancing hydrophilicity. At the same time, it could improve the cell affinity on the surface of the material, which was beneficial to increase the cell adhesion rate and cell activity, promote the osteogenic differentiation of human mesenchymal stem cells grown on the materials, and improve the alkaline phosphatase activity, calcium nodules, and the expression of related osteogenic genes and proteins. These results demonstrated potential applications of PU/LC composite scaffolds in repairing or regeneration of bone tissue engineering.