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Short communication: Algicide activity of antimicrobial peptides compounds against Prototheca bovis

V R Sperotto1, L B Denardi2, C Weiblen3

  • 1Pharmacology Postgraduate Program, Federal University of Santa Maria, Rio Grande do Sul, Brazil, 97105-900; University of Cruz Alta, Cruz Alta, Rio Grande do Sul, Brazil, 98005-972.

Journal of Dairy Science
|January 18, 2021
PubMed

Insights

Antimicrobial peptides like pexiganan show promise in controlling Prototheca bovis, a cause of bovine mastitis. These peptides offer a novel treatment strategy against this challenging veterinary pathogen.

Area of Science:

  • Veterinary Microbiology
  • Antimicrobial Peptides
  • Algal Pathogens

Background:

  • Prototheca bovis is a significant etiological agent of bovine mastitis.
  • Existing treatments for Prototheca bovis infections are limited.
  • Antimicrobial peptides (AMPs) represent a potential alternative therapeutic class.

Purpose of the Study:

  • To evaluate the in vitro efficacy of selected antimicrobial peptides against Prototheca bovis.
  • To determine the minimum inhibitory concentrations (MICs) and algicidal effects of these peptides.

Main Methods:

  • In vitro susceptibility testing of Prototheca bovis isolates (n=32).
  • Evaluation of six antimicrobial peptides: pexiganan (MSI-78), h-Lf1-11, LL-37, cecropin B, magainin-2, and fengycin B.
  • Determination of Minimum Inhibitory Concentration (MIC) and algicidal activity.

Main Results:

  • Pexiganan, h-Lf1-11, LL-37, and cecropin B demonstrated significant inhibitory and algicidal activity against Prototheca bovis.
  • MIC values ranged from 5-10 µg/mL for pexiganan and 5-20 µg/mL for algicidal effect.
  • MICs for h-Lf1-11, LL-37, and cecropin B were 10-80 µg/mL, 20-80 µg/mL, and 40-160 µg/mL, respectively.

Conclusions:

  • Selected antimicrobial peptides, particularly pexiganan, show potent in vitro activity against Prototheca bovis.
  • These findings suggest a promising novel therapeutic approach for managing bovine mastitis caused by Prototheca bovis.
  • Further research into AMPs could lead to new treatments for veterinary mastitis.
Keywords:
antimicrobial peptidescecropin Bh-Lf1-11protothecosis

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